Vaccine Adjuvants

1. Overview of Vaccine Adjuvants: Introduction, History, and Current Status            Ruchi R. Shah, Kimberly J. Hassett, and Luis A. Brito2. Development of the CpG Adjuvant 1018: A Case Study            John D. Campbell3. Syntheses of Human TLR8-Specific Small-Molecule Agonists            Mallesh Beesu, Hari Prasad Kokatla, and Sunil A. David4. Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21            Alberto Fernández-Tejada, William E. Walkowicz, Derek S. Tan, and David Y. Gin5. QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes            Livia Brunner, Christophe Barnier-Quer, and Nicolas Collin6. Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography            Anna C.A. Yendo, Fernanda de Costa, Carla Kauffmann, Juliane D. Fleck, Grace Gosmann, and Arthur G. Fett-Neto7. Biosynthetic Approaches to Squalene Production: The Case of Yeast            Martin Valachovic and Ivan Hapala8. In Silico Adjuvant Design and Validation            Matthew N. Davies, Helene Pere, Iris Bosschem, Freddy Haesebrouck, Bram Flahou, Eric Tartour, Darren R. Flower, David F. Tough, and Jagadeesh Bayry9. Manufacturing Methods for Liposome Adjuvants             Yvonne Perrie, Elisabeth Kastner, Swapnil Khadke, Carla B. Roces, and  Peter Stone10. Synthesis of Lymph Node-Targeting Adjuvants            Melissa C. Hanson and Darrell J. Irvine11. Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination            Manjari Lal, Jessica White, and Changcheng Zhu12. Manufacture of Oil-in-Water Emulsion Adjuvants            Jean Haensler13. Methods to Prepare Aluminum Salt-Adjuvanted Vaccines            Sachin G. Thakkar and Zhengrong Cui14. Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines            Cristina Maria de Barros, Emad Ibrahim Wafa, Khanidtha Chitphet, Kawther Ahmed, Sean M Geary, and Aliasger K. Salem15. Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product            Michelle Y. Chan, Timothy S. Dutill, and Ryan M. Kramer16. Stressed Stability Techniques for Adjuvant Formulations            Manvi Hasija, Anthony Sheung, Nausheen Rahman, and Salvador F. Ausar17. Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA)            Michelle Y. Chan, Quinton M. Dowling, Sandra J. Sivananthan, and Ryan M. Kramer18. Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling            Quinton M. Dowling and Ryan M. Kramer 19. Determination of Protein Content in Alhydrogelâ-Based Vaccines by O-Phthalaldehyde Assay            Kelly M. Rausch and Daming Zhu20. Staining and Transfer Techniques for SDS-PAGE Gels to Minimize Oil-in-Water Emulsion Adjuvant Interference            Alicia M. Schwartz, Michelle Y. Chan, Dawn M. Fedor, Sandra J. Sivananthan, and Ryan M. Kramer21. Interactions Between Antigens and Nanoemulsion Adjuvants: Separation and Characterization Techniques            Michelle Y. Chan, Dawn M. Fedor, Tony Phan, Lucien Barnes V, and Ryan M. Kramer22. Screening Vaccine Formulations in Fresh Human Whole Blood            Jalil Hakimi, Sepideh Aboutorabian, Frederick To, Salvador F. Ausar, Nausheen Rahman, and Roger H. Brookes23. Analysis of the Innate Response to Adjuvants: Characterization of the Draining Lymph Node by Fluorescence-Activated Cell Sorting            Anthony L. Desbien 24. Assessment of Antigen-Specific Cellular Immunogenicity Using Intracellular Cytokine Staining, ELISpot, and Culture Supernatants            Elyse A. Beebe and Mark T. Orr25. Eliciting Epitope-Specific CD8 T Cell Response by Immunization with Microbial Protein Antigens Formulated with -Galactosylceramide: Theory, Practice, and Protocols            Pavlo Gilchuk, Frances C. Knight, John T. Wilson, and Sebastian Joyce26. Molecular Methods and Bioinformatic Tools for Adjuvant Characterization by High-Throughput Sequencing            Steven R. Wiley and Vanitha S. Raman... Mehr