The Complement System

1. OverviewMihaela Gadjeva 2. Evaluation of Complement Function by ELISAAnja Roos and Jorgen Wieslander3. Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization. Mihaela Kojouharova 4. Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional CharacterizationVéronique Rossi, Isabelle Bally, Monique Lacroix, Gérard J Arlaud, and Nicole M Thielens 5. Expression and Purification Methods for the Production of Recombinant Human Complement Component C2 Angela W. Norton, Paolo G.V. Martini, Lynette C. Cook, Scott Alderucci, Dianna M. Lundberg, Susan M. Fish, Charles Bedard3, John Gill, Arthur O. Tzianabos, and Michael F. Concino 6. Purification and Characterisation of human and Mouse Complement C3Marieta M Ruseva and Meike Heurich 7. Purification of Human Complement Protein C5Lars Sottrup-Jensen and Gregers Rom Andersen 8. Quantification of Complement C5b-9 Binding to Cells by Flow CytometryOren Moskovich and Zvi Fishelson 9. Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding LectinThomas Vorup-Jensen 10. Genotyping of FCN and MBL2 Polymorphisms using PyrosequencingLea Munthe-Fog, Hans Ole Madsen, and Peter Garred 11. Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement SystemTroels R. Kjaer and Steffen Thiel 12. Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins Misao Matsushita, David Kilpatrick, Hiroshi Shiraki, Yu Liu, Koichiro Tateishi, Mitsushi Tsujimura, Yuichi Endo, Teizo Fujita 13. Purification and Functional Analysis of Human ProperdinJoseph O`Flynn, Maria C. Faber Krol, Mohamed R. Daha, Cees van Kooten 14. Purification and Functional Characterization of C4b-binding Protein (C4BP)Frida C. Mohlin and Anna M. Blom 15. Purification and Functional Characterization of Factor ISara C. Nilsson and Anna M. Blom 16. C1 inhibitor - Quantification and PurificationLilian Varga and József Dobó 17. Purification, Quantification and Functional Analysis of Complement Factor H Bing-Bin Yu, Beryl E. Moffatt, Marina Fedorova, Ali Ryan, Claire G.S. Villiers, James N. Arnold, Eugenie Du, Astrid Swinkels, Man Chung Li, Robert B. Sim 18. Factor H-related ProteinsMihály Józsiand Seppo Meri 19. Functional Evaluation of Factor H genetic and Acquired Abnormalities, Application for Atypical Hemolytic Uremic Syndrome (aHUS)Lubka T. Roumenina, Roxane Roquigny, Caroline Blanc, Nelly Poulain, Stephanie Ngo, Marie-Agnes Dragon-Durey, and Veronique Fremeaux-Bacchi 20. Anti-Factor H Auto Antibodies AssayMarie-Agnès Dragon-Durey, Caroline Blanc, Lubka Roumenina, Nelly Poulain Poulain, Stéphanie Ngo, Pauline Bordereau, Véronique Frémeaux-Bacchi 21. Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage Najmunisa Karbani, Eswari Dodagatta-Marri, Asif S. Qaseem, Priyaa Madhukaran, Patrick Waters, Anthony G. Tsolaki, Taruna Madan, and Uday Kishore 22. Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar LavageEswari Dodagatta-Marri, Asif Qaseem,Najmunisa Karbani, Anthony George Tsolaki, Patrick Waters, Taruna Madan, and Uday Kishore 23. Functional Analysis of C5a Effector Responses In Vitro and In Vivo Christian M. Karsten, Yves Laumonnier,and Jörg Köhl 24. Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow CytometryLuke R Donius and John H Weis 25. Quantification of Complement Receptor 2 Calcium Signaling Enhancement Using Flow CytometryLuke R Donius and John H Weis 26. Purification of C1q Receptors and Functional AnalysisBerhane Ghebrehiwet and Ellinor I.B. Peerschke 27. Detection of Cell Membrane-bound CD46 using Flow CytometryMartin Kolev and Claudia Kemper 28. Detection of CD59 using Flow CytometryMartin Kolev 29. Functional Analysis of CD59 using Complement Dependent Cytotoxicity AssayMartin Kolev 30. Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry Gitte Krogh Nielsenand Thomas Vorup-Jensen 31. Analysis of the Complement Activation in MiceNirmal K. Banda and Kazue Takahashi 32. Opsonophagocytic AssayMarkryan Dwyer and Mihaela Gadjeva... Mehr