T-Cell Motility

Preface...Table of Contents...Contributing Authors...1. An Introduction to LFA-1/ICAM-1 Interactions in T-Cell MotilityNavin Kumar Verma and Dermot Kelleher2. Isolation of Human Peripheral Blood T LymphocytesAtish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma3. A Laboratory Model to Study T-Cell MotilityNavin Kumar Verma, Sjyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher4. Quantification of T-Cell Migratory Phenotypes using High Content AnalysisAik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma5. Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-TimeSeow Theng Ong and Navin Kumar Verma6. Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-CellsSeow Theng Ong, Graham D. Wright, and Navin Kumar Verma7. Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell ChemotaxisPraseetha Prasannan and Navin Kumar Verma8. Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-LymphocytesAtish Kizhakeyil and Navin Kumar Verma9. GapmeR-Mediated Gene Silencing in Motile T-CellsMobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Chalasani, Atish Kizhakeyil, and Navin Kumar Verma10. Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal MicroscopyEmmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann11. Phosphoprotein Enrichment for Protein Analysis in Motile T-LymphocytesPraseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma12. Immunometabolomic Phenotyping of Motile T-CellsMadhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma13. Profiling Activity of Cellular Kinases in Migrating T-CellsChandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and Wim Vanden Berghe14. Biotinylation Assay to Determine LFA-1 Recycling in Motile T-LymphocytesMalin Samuelsson and Lena M. Svensson15. A Protocol to Study T-Cell Signalling in an Immune Synapse by MicroscopyMobashar Hussain Urf Turabe Fazil, Pankal Kumar, and Navin Kumar Verma16. Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic PeptidesMadhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma17. Modified Intravital Microscopy to Assess Vascular Health and T-Cell MotilityGeoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers18. Computational Analysis of Protein-Protein Interactions in Motile T-CellsSunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khursheed Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma... Mehr