Part I: Proteoglycans 1. Proteoglycans: Gene Cloning Mauricio Cortes, James R. Mensch, Miriam Domowicz, and Nancy B. Schwartz 2. Proteoglycan: Site Mapping and Site-Directed Mutagenesis Fred K. Hagen 3. Mapping of the Wnt/beta-Catenin/TCF Response Elements in the Human Versican Promoter Maziar Rahmani, Jon M. Carthy, and Bruce M. McManus 4. Gene Silencing in Mouse Embryonic Stem Cells Norihiko Sasaki and Shoko Nishihara 5. A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout María T. Dours-Zimmermann and Dieter R. Zimmermann 6. Detection of Neurocan in Cerebrospinal Fluid Uwe Rauch Part II: Glycosaminoglycans Analysis 7. Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization) Shuji Mizumoto and Kazuyuki Sugahara 8. Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis Nathalie Mainreck, Stéphane Brézillon, Ganesh D. Sockalingum, François-Xavier Maquart, Michel Manfait, and Yanusz Wegrowski 9. Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography/Specific Labeling Derek J. Langelsay, Christopher J. Jones, Szabolcs Beni, and Cynthia K. Larive 10. Brain Chondroitin/Dermatan Sulfate, from Cerebral Tissue to Fine Structure: Extraction, Preparation, and Fully Automated Chip-Electrospray Mass Spectrometric Analysis Alina D. Zamfir, Corina Flangea, Alina Serb, Eugen Sisu, Leon Zagrean, Andreas Rizzi, and Daniela G. Seidler 11. Use of Neutrons Reveal the Dynamics of Cell Surface Glycosaminoglycans Marion Jasnin 12. Following Protein-Glycosaminoglycan (GAG) Polysaccharide Interactions with Differential Scanning Fluorimetry (DSF) Katarzyna A. Uniewicz, Alessandro Ori, Timothy R. Rudd, Marco Guerrini, Mark C. Wilkinson, David G. Fernig, and Edwin A. Yates 13. In Vivo Scintigraphic Imaging of Proteoglycans Elisabeth Miot-Noirault, Aurélien Vidal, Philippe Auzeloux, Caroline Peyrode, Jean-Claude Madelmont, and Jean-Michel Chezal Part III: PGs: Multifunctional Cell Regulators 14. Serglycin: The Master of the Mast Cell Elin Rönnberg and Gunnar Pejler 15. Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry Peter J. Roughley and John S. Mort 16. Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors Jin-ping Li and Dorothe Spillmann Part IV: Proteoglycans Involvement in Pathophysiology 17. Models for Studies of Proteoglycans in Kidney Pathophysiology Scott J. Harvey 18. Lumican Promotes Corneal Epithelial Wound Healing Chia-Yang Liu and Winston Whei-Yang Kao 19. Shedding of Cell Membrane-Bound Proteoglycans Eon Jeong Nam and Pyong Woo Park 20. Modulatory Effects of Proteoglycans on Proteinase Activities Steven Georges, Dominique Heymann, and Marc Padrines 21. Proteoglycans and Osteolysis Marc Baud'Huin, Céline Charrier, Gwenola Bougras, Régis Brion, Frédéric Lezot, Marc Padrines, and Dominique Heymann 22. Proteoglycans and Cartilage Repair Mohamed Ouzzine, Narayanan Venkatesan, and Sylvie Fournel-Gigleux... Mehr