Protein Misfolding Diseases

Part I: Protein Biophysics Assays 1. Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation            Joana S. Cristóvão, Bárbara J. Henriques, and Cláudio M. Gomes 2. Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, -Synuclein, and Prion Aggregates            Eri Saijo, Bradley R. Groveman, Allison Kraus, Michael Metrick, Christina D. Orrù, Andrew G. Hughson, and Byron Caughey 3. Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides            Ravit Malishev, Sofiya Kolusheva, and Raz Jelinek 4. Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis            Naoto Soya, Ariel Roldan, and Gergely L. Lukacs 5. A Multipronged Method for Unveiling Subtle Structural-Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies            Donatella Bulone, Pier Luigi San Biagio, Tatiana Quiñones-Ruiz, Manuel Rosario-Alomar, Igor K. Lednev, Frank T. Robb,Everly Conway de Macario, and Alberto J.L. Macario 6. High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1            Alireza Abdolvahabi, Sanaz Rasouli, Corbin M. Croom, and Devon L. Plewman 7. Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases            Devkee M. Vadukul, Youssra K. Al-Hilaly, and Louise C. Serpell 8. Assays for Light Chain Amyloidosis Formation and Cytotoxicity            Luis M. Blancas-Mejia, Pinaki Misra, Christopher J. Dick, Marta Marin Argany, Keely R. Redhage, Shawna A. Cooper, and Marina Ramirez-Alvarado Part II: Cellular And Proteostasis Assays 9. Monitoring Aggregate Clearance and Formation in Cell-Based Assays            Evelien Eenjes, Young Joo Yang-Klingler, and Ai Yamamoto 10. Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor            Yu Liu, Matthew Fares, and Xin Zhang 11. Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation            Vibha Prasad and Aaron Voigt 12. Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry            Salvador Ventura and Susanna Navarro 13. Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells            Edward Pokrishevsky, Jeremy Nan, and Neil R. Cashman 14. A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction            Cagla Cömert, Paula Fernandez Guerra, and Peter Bross 15. Super-Resolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells            Steffen J. Sahl and Willianne I.M. Vonk Part III: Protein Folding Recovery and Correction Assays 16. Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry            Tânia G. Lucas, Cláudio M. Gomes, and Bárbara J. Henriques 17. Methods to Screen Compounds against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics            Giulia Diniz da Silva Ferretti, Danielly Ferraz da Costa, Jerson Lima da Silva, and Luciana Pereira Rangel 18. Early Stage Discovery and Validation of Pharmacological Chaperones for the Correction of Protein Misfolding Diseases            Oscar Aubi, Per M. Knappskog, and Aurora Martinez 19. Constructing Kinetically Controlled Denaturation Isotherms of Folded Proteins Using Denaturant-Pulse Chaperonin Binding            Pierce T. O'Neil, Alexandra J. Machen, Jackie A. Thompson, Wei Wang, Quyen Q. Hoang, Michael R. Baldwin, Karen R. Khar, John Karanicolas, and Mark T. Fisher 20. In Vitro Prion Amplification Methodology for Inhibitor Screening            Tuane Cristine R.G. Vieira and Jerson L. Silva 21. SolubiS: Optimizing Protein Solubility by Minimal Point Mutations            Rob van der Kant, Joost van Durme, Frederic Rousseau, and Joost Schymkowitz... Mehr