Protein Downstream Processing

Part I. Screening and Design Purification Strategies 1. Protein Purification: An OverviewNikolaos Labrou 2. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoEIgnacio Amadeo, Laura Mauro, Eduardo Ortí, and Guillermina Forno 3. High Throughput Process Development: I. Process ChromatographyA. S. Rathore and R. Bhambure 4. High Throughput Process Development: II. Membrane ChromatographyA. S. Rathore and S. Muthukumar 5. Media Selection in Ion Exchange Chromatography in a Single MicroplateCharlotte Cabanne and Xavier Santarelli 6. High Throughput Screening of Dye-Ligands for ChromatographySunil Kumar and Narayan S. Punekar 7. Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette TipsSunil Chhatre and Daniel G. Bracewell Part II. Low-Resolution Protein Purification Methods 8. Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline Zhengrong GU 9. Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from PlantsOscar Aguilar and Marco Rito-Palomares 10. Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic ProteinsA. S. Rathore and R. Bhambure 11. Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP)Ipsita Roy, Kalyani Mondal, and Munishwar N. Gupta 12. Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and PeriplasmArio de Marco 13. Immunoglobulin Purification by Caprylic AcidVictor Morais, Patricia Berasain, and Hugo Massaldi Part III: High-Resolution Protein Purification Methods 14. Affinity Tags in Protein Purification and Peptide Enrichment: An OverviewAna Sofia Pina, Íris L. Batalha, and Ana Cecília A. Roque 15. Expression and Purification of Large Active GST Fusion EnzymesStefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, and Paola Loguercio Polosa 16. Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion ProteinsSharon Williams, Phil Morton, and Dev Baines 17. Zbasic - A Purification Tag for Selective Ion-Exchange RecoveryMy Hedhammar, Johan Nilvebrant, and Sophia Hober 18. An Orthogonal Fusion Tag for Efficient Protein PurificationJohan Nilvebrant, Mikael Åstrand, and Sophia Hober 19. Phage Display of Engineered Binding ProteinsMark Levisson, Ruud B. Spruijt, Ingrid Nolla Winkel, Servé W.M. Kengen, and John van der Oost 20. Biomimetic Affinity Ligands for Protein PurificationIsabel T. Sousa and M. Ângela Taipa 21. Synthesis and Application of Dye-Ligand Affinity AdsorbentsEvangelia Chronopoulou and Nikolaos E. Labrou 22. Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein PurificationS. A. Camperi, M. C. Martínez-Ceron, S. L. Giudicessi, M. M. Marani, F. Albericio, and O. Cascone 23. Affinity Chromatography of Proteins on Monolithic ColumnsE.G. Vlakh, G.A. Platonova, and T.B. Tennikova 24. Sample Displacement Batch Chromatography of ProteinsMarta Kotasinska, Verena Richter, Marcel Kwiatkowski, and Hartmut Schlüter Part IV. Assessing Protein Structural Integrity, Purity, and Stabilization 25. Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS ApproachesCatalin E. Doneanu, Weibin Chen, and St John Skilton 26. Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography ApplicationsJens Sproß and Andrea Sinz 27. Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass SpectrometryCosima Damiana Calvano, Cristina De Ceglie, and Carlo G. Zambonin 28. Modern Bioanalysis of Proteins by Electrophoretic Techniques Sona Krizkova, Marketa Ryvolova, Michal Masarik, Ondrej Zitka, Vojtech Adam, Jaromir Hubalek, Tomas Eckschlager, and Rene Kizek 29. Protein Structure Validation and Analysis with X-Ray Crystallography Anastassios C. Papageorgiou and Jesse Mattsson 30. Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized EnzymesAnnette C. Moser, Benjamin White, and Frank A. Kovacs 31. Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An OverviewKen-ichi Izutsu 32. Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of TubulinPavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, and Eduarda Dráberová Part V. Applications/Case Studies 33. G Protein-Coupled Receptor Expression and PurificationKarolina Corin, Lotta T. Tegler, and Sotirios Koutsopoulos 34. (Hyper)thermophilic Enzymes: Production and PurificationPierpaolo Falcicchio, Mark Levisson, Servé W.M. Kengen, Sotirios Koutsopoulos , and John van der Oost 35. Affinity Chromatography for Antibody PurificationSushrut Arora, B. Vijayalakshmi Ayyar, and Richard O'Kennedy 36. Screening and Purification of Recombinant Lignocellulolytic EnzymesEvangelos Topakas and Paul Christakopoulos 37. Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFvAnna Moosmann, Egbert Müller, and Heiner Böttinger 38. One-Step Purification of Glutamate decarboxylase from E. coli Using Aqueous Two Phase SystemW. Yao, J. Zhu, and B. Sun... Mehr