Protein Chromatography

Part I Overview Chapters1. A Synopsis of Protein and their PurificationDermot Walls, Gary Cooney, and Sinéad T. Loughran2. Gel-Filtration ChromatographyCiarán Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor3. Immunoaffinity Chromatography: Concepts and ApplicationsJenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O'Kennedy4. Avoiding Proteolysis During Protein PurificationBarry J. Ryan and Gary T. Henehan5. Scale-Up of Protein Purification: Downstream Processing IssuesJohn Joseph Milne6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune SourcesWilliam J.J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ní Shúilleabháin, and Orla Cunningham7. Protein Stability: Enhancement and MeasurementCiarán Ó'Fágáin8. Tagging Recombinant Proteins to Enhance Solubility and Aid PurificationSinéad T. Loughran and Dermot WallsPart II Protocols Chapters9. Storage and Lyophilisation of Pure ProteinsCiarán Ó'Fágáin and Keith Colliton10. Differential Precipitation and Solubilization of ProteinsBarry J. Ryan and Gemma K. Kinsella11. Ion-Exchange Chromatography: Basic Principles and ApplicationPhilip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor12. Protein Quantitation and Analysis of PurityEva M. Campion, Sinéad T. Loughran, and Dermot Walls13. Purification of Proteins Fused to Maltose-Binding ProteinMario Lebendiker and Tsafi Danieli14. Purification of Poly-Histidine-Tagged ProteinsSinéad T. Loughran, Ronan T. Bree, and Dermot Walls15. Purification of Antibodies Using Affinity ChromatographyElaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O' Kennedy16. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized ChickensWilliam J.J. Finlay, Laird Bloom, Sreeja Varghese, Bénédicte Autin, and Orla Cunningham17. Measuring Protein-Protein Interactions Using BiacorePaul Leonard, Stephen Hearty, Hui Ma, and Richard O'Kennedy18. Hydrophobic Interaction ChromatographyBrendan F. O'Connor and Philip M. Cummins19. Fast Protein Liquid ChromatographyAshkan Madadlou, Siobhan O'Sullivan, and David Sheehan20. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification SystemsMichael Henry and Paula Meleady21. Strategies for the Purification of Membrane ProteinsSinéad M. Smith22. Antimicrobial Peptide Production and PurificationSrinivas Suda, Des Field, and Niall Barron 23. Lectin Affiity Chromatography (LAC)`Brendan F. O'Connor, Donal Monaghan, and Jonathan Cawley... Mehr