Xylose/Glucose isomerase is an important industrial enzyme, which is being used for the conversion of glucose into fructose for decades. This research focuses on the production of glucose isomerase from Escherichia coli DH5a. Fermentation optimization studies showed that higher amount of yeast extract and tryptone increased the cell growth and the optimum temperature for growth was 37°C. Partial purification of xylose isomerase was carried out using heat treatment and ammonium sulphate precipitation.... Mehr