1. Assessing Metabolic Flux in Plants with RadiorespirometryNicholas J. Kruger, Shyam K. Masakapalli, and R. George Ratcliffe 2. Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory FluxesToshihiro Obata, Laise Rosado-Souza, and Alisdair R. Fernie 3. A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution Hardy Rolletschek and Gregor Liebsch 4. VisiSens Technique to Measure Internal Oxygen and Respiration in Barley RootsAprajita Kumari and Kapuganti Jagadis Gupta 5. MultiSense: A Multimodal Sensor Tool Enabling the High Throughput Analysis of RespirationPeter Keil, Gregor Liebsch, Ljudmilla Borisjuk, and Hardy Rolletschek 6. Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds using Micro SensorSonika Pandey, Aprajita Kumari, Bharadwaj C, and Kapuganti Jagadis Gupta 7. Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringe InfectionPradeep Kumar Pathak and Kapuganti Jagadis Gupta 8. Measurement of Oxygen Status in Arabidopsis Leaves Undergoing Hypersensitive Response during Pseudomonas InfectionAprajita Kumari, Gail Preston, and Kapuganti Jagadis Gupta 9. Isolation of Physiologically Active and Intact Mitochondria from ChickpeaSonika Pandey, Aprajita Kumari, and Kapuganti Jagadis Gupta 10. Isolation and Structural Studies of Mitochondria from Pea Roots Abhaypratap Vishwakarma and Kapuganti Jagadis Gupta 11. Mitochondrial Respiration and Oxygen TensionDaniel S. Shaw, Karlia Meitha, Michael J. Considine, and Christine H. Foyer12. Isolation of Mitochondria from Model and Crop PlantsSandra M. Kerbler and Nicolas L. Taylor 13. Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in FruitsJurandi Gonçalves de Oliveira, Luis Miguel Mazorra Morales, Gláucia Michelle Cosme Silva, Kátia Daniella da Cruz Saraiva, Diederson Bortolino Santana, Clesivan Pereira dos Santos, Marcos Goes Oliveira, and José Hélio Costa 14. Measurement of Tricarboxilic Acid Cycle Enzyme Activities in Plants Rebeca Patricia Omena-Garcia, Wagner L. Araújo, Yves Gibon, Alisdair R. Fernie, and Adriano Nunes-Nesi 15. Respiration Traits as Novel Markers for Plant Robustness under the Threat of Climate Change - A Protocol for Validation Birgit Arnholdt-Schmitt 16. Calorespirometry - A Novel Tool in Functional Hologenomics to Select 'Green' Holobionts for Biomass ProductionBirgit Arnholdt-Schmitt and Vinod Kumar Patil 17. Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant TissuesNestor Fernandez Del-Saz, Miquel Ribas-Carbo, Gabriel Martorell, Alisdair R. Fernie, and Igor Florez-Sarasa 18. A Driving Bioinformatics Approach to Explore Co-regulation of AOX Gene Family Members during Growth and DevelopmentJosé Hélio Costa and Birgit Arnholdt-Schmitt 19. A Step-by-Step Protocol for Classifying AOX Proteins in Flowering PlantsJosé Hélio Costa, Clesivan Pereira dos Santos, Kátia Daniella da Cruz Saraiva, and Birgit Arnholdt-Schmitt 20. Studying Individual Plant AOX Gene Functionality in Early Growth Regulation - A New ApproachBirgit Arnholdt-Schmitt and Vinod Kumar Patil 21. Laser-Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L.Carla Ragonezi and Birgit Arnholdt-Schmitt 22. Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative PathwaysBobba Sunil and Agepati S. Raghavendra 23. Measuring Spatial and Temporal Oxygen Flux near Plant Tissues using a Self-Referencing Optrode Eric S. McLamore, D.M. Porterfield, and Yinglang Wan... Mehr