Peroxisomes

1. Isolation of Peroxisomes from Rat Liver and Cultured Hepatoma Cells by Density Gradient CentrifugationAndreas Manner and Markus Islinger 2. Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney PeroxisomesMiriam J. Schönenberger and Werner J. Kovacs 3. Determining the Topology of Peroxisomal Proteins Using Protease Protection AssaysTânia Francisco, Ana F. Dias, Ana G. Pedrosa, Cláudia P. Grou, Tony A. Rodrigues, and Jorge E. Azevedo 4. Isolation of Native Soluble and Membrane-Bound Protein Complexes from Yeast Saccharomyces CerevisiaeTobias Hansen, Anna Chan, Thomas Schröter, Daniel Schwerter, Wolfgang Girzalsky, and Ralf Erdmann 5. Method for Measurement of Peroxisomal Very Long-Chain Fatty Acid Beta-Oxidation and de novo C26:0 Synthesis Activity in Living Cells Using Stable-Isotope Labelled Docosanoic AcidMalu-Clair van de Beek, Inge M.E. Dijkstra, and Stephan Kemp 6. Analysis of Plasmalogen Synthesis in Cultured CellsMasanori Honsho and Yukio Fujiki 7. Transfection of Primary Human Skin Fibroblasts for Peroxisomal StudiesJanet Koster and Hans R. Waterham 8. siRNA-Mediated Silencing of Peroxisomal Genes in Mammalian CellsTina A. Schrader and Michael Schrader 9. Dual Reporter Systems for the Analysis of Translational Readthrough in MammalsJulia Hofhuis, Severin Dieterle, Rosemol George, Fabian Schueren, and Sven Thoms 10. Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3'-diaminobenzidineH. Dariush Fahimi 11. Cryo-Immuno Electron Microscopy of Peroxisomal Marker ProteinsKarina Mildner and Dagmar Zeuschner 12. Detection and Immunolabelling of Peroxisomal ProteinsTina Schrader, Markus Islinger, and Michael Schrader 13. Labelling of Peroxisomes for Live Cell Imaging in the Filamentous Fungus Ustilago MaydisSofia C. Guimaraes, Sreedhar Kilaru, Michael Schrader, and Martin Schuster 14. Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-based ProbesCelien Lismont, Paul A. Walton, and Marc Fransen 15. KillerRed as a Tool to Study the Cellular Responses to Peroxisome-Derived Oxidative StressMarc Fransen and Chantal Brees 16. Determination of Peroxisomal pH in Living Mammalian Cells Using pHRedLuis F. Godinho, and Michael Schrader 17. In Cellulo Approaches to Study Peroxisomal Protein Import - Yeast Immunofluorescence MicroscopyTobias Hansen, Wolfgang Girzalsky, and Ralf Erdmann 18. Blue Native Page: Applications to Study Peroxisome BiogenesisKanji Okumoto, Shigehiko Tamura, and Yukio Fujiki 19. In Vitro PMP Import Analysis Using Cell-Free Synthesized PMP and Isolated PeroxisomesYuqiong Liu, Masanori Honsho, and Yukio Fujiki 20. Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell SystemKanji Okumoto, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki 21. The Use of Glycosylation Tags as Reporters for Protein Entry into the Endoplasmic Reticulum in Yeast and Mammalian CellsJudith Buentzel, and Sven Thoms 22. Detection of Ubiquitinated Peroxisomal Proteins in YeastNatasha Danda and Chris Williams 23. Assessing Pexophagy in Mammalian CellsShun-ichi Yamashita and Yukio Fujiki 24. Experimental Systems to Study Yeast PexophagyShun-ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, and Yukio Fujiki 25. Flow Cytometric Analysis of the Expression Pattern of Peroxisomal Proteins, Abcd1, Abcd2, and Abcd3 in BV-2 Murine Microglial CellsThomas Nury, Imen Helali, El Mostafa Karym, Meryam Debbabi, Flore Geillon, Catherine Gondcaille, Doriane Trompier, Amina Najid, Sébastien Terreau, Maryem Bezine, Amira Zarrouk, Anne Vejux, Pierre Andreoletti, Mustapha Cherkaoui-Malki, Stéphane Savary, and Gérard Lizard 26. Study of Peroxisomal Protein Phosphorylation by Functional ProteomicsAndreas Schummer, Sven Fischer, Silke Oeljeklaus, and Bettina Warscheid 27. Analysis of Peroxisomal beta-Oxidation during Storage Oil Mobilization in Arabidopsis Thaliana SeedlingsBjörn Hielscher, Lennart Charton, Tabea Mettler-Altmann, and Nicole Linka 28. Peroxisome Mini-Libraries - Systematic Approaches to Study Peroxisomes Made EasyNoa Dahan, Maya Schuldiner, and Einat Zalckvar 29. Generation of Peroxisome-Deficient Somatic Animal Cell MutantsKanji Okumoto and Yukio Fujiki 30. Clinical and Laboratory Diagnosis of Peroxisomal DisordersRonald J.A. Wanders, Femke C.C. Klouwer, Sacha Ferdinandusse, Hans R. Waterham, and Bwee Tien Poll-Thé... Mehr