1. Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral NeuronsLaura Lossi, Claudia Castagna, and Adalberto Merighi Part I: Molecular, Sturctural, Functional, and Genomic Changes in Dying Neurons2. Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic MicroscopyNicolas Pavillon and Pierre Marquet 3. Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei Noelia López-Sánchez and José M. Frade 4. Nuclear Signs of Pre-NeurodegenerationFernando C. Baltanás, Jorge Valero, Jose Ramón Alonso, Maria Teresa Berciano, and Miguel Lafarga 5. Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent ProbesRuslan I. Dmitriev and Dmitri B. Papkovsky 6. Calcium Imaging in Neuron Cell DeathMaría Calvo, Carlos Villalobos, and Lucía Núñez 7. Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy AnalysisMacarena S. Arrázolaand Nibaldo C. Inestrosa 8. Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET)Silvia Alasia, Carolina Cocito, Adalberto Merighi, and Laura Lossi 9. Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to Study the Function of Selected Proteins in Neuronal ApoptosisNadia Canu 10. Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina Sook Hyun Chung, Weiyong Shen, and Mark Gillies 11. Genomic Analysis of Transcriptional Changes Underlying Neuronal ApoptosisSebastiano Cavallaro 12. High-Throughput Cell Death AssaysMatthew E. Pamenter and Gabriel G. Haddad PartII: Cell Death in Neuropathology and Experimental Neuoropathology 13. Staining of Dead Neurons by the Golgi Method in Autopsy MaterialStavros J. Baloyannis 14. Image Analysis Algorithms for Immunohistochemical Assessment of Cell DeathStan Krajewski, Jeffrey Wang, Tashmia Khan, Jonathan Liu, Chia-Hung Sze, and Maryla Krajewska 15. In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell DeathCarla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti 16. Laser Microbeam Targeting of Single Nerve Axons in Cell CultureNicholas Hyun, Linda Z. Shi, and Michael W. Berns 17. Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser OphthalmoscopyEduardo M. Normando, Mohammad H. Dehabadi,Li Guo, Lisa A. Turner, Gaia Pollorsi, and M. Francesca Cordeiro 18. Targeted Toxicants to Dopaminergic Neuronal Cell DeathHuajun Jin, Arthi Kanthasamy, Dilshan S. Harischandra, Vellareddy Anantharam, Ajay Rana, and Anumantha G. Kanthasamy Part III: Neural Stem Cells, Progenitors, and Gene Therapy Strategies19. Stem Cells, Neural Progenitors, and Engineered Stem CellsRaj R. Rao and Shilpa Iyer 20. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene TherapyPeggy Marconi, Cornel Fraefel,and Alberto L. Epstein 21. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene TherapyCornel Fraefel, Peggy Marconi, and Alberto L. Epstein 22. Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous SystemDavid Díaz, José Ramón Alonso, and Eduardo Weruaga Part IV: Neuronal Death in Non Mammalian Models23. Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA)Antón Barreiro-Iglesias and Michael I. Shifman 24. Generation of Zebrafish Models by CRISPR/Cas9 Genome EditingAlexander Hruscha and Bettina Schmid 25. In Vivo Assessment of Neuronal Cell Death in DrosophilaPierre Dourlen 26. Drosophila Model for Studying Phagocytosis Following Neuronal Cell DeathBoris Shklyar, Flonia Levy-Adam, and Estee Kurant... Mehr