Insoluble Proteins

1. General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins Neus Ferrer-Miralles, Paolo Saccardo, José Luis Corchero, Zhikun Xu, and Elena García-Fruitós Part I: Recombinant Protein Production in Escherichia coli 2. Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production Agustín Correa and Pablo Oppezzo 3. Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility Ranjana Arya, Jamal S.M. Sabir, Roop S. Bora, and Kulvinder S. Saini 4. Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification Zhanglin Lin, Qing Zhao, Bihong Zhou, Lei Xing, and Wanghui Xu 5. Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them Jason T. Boock, Dujduan Waraho-Zhmayev, Dario Mizrachi, and Matthew P. DeLisa 6. Characterization of Amyloid-Like Properties in Bacterial Intracellular Aggregates Anna Villar-Pique, Susanna Navarro, and Salvador Ventura Part II: Strategies to Produce Insoluble Proteins in Cell-Free Expression Systems 7. Co-Translational Stabilization of Insoluble Proteins in Cell-Free Expression Systems Lei Kai, Erika Orbán, Erik Henrich, Davide Proverbio, Volker Dötsch, and Frank Bernhard Part III: Recombinant Protein Production in Lactic Acid Bacteria (LAB) 8. Functional Expression of Plant Membrane Proteins in Lactococcus lactis Sylvain Boutigny, Emeline Sautron, Annie Frelet-Barrand, Lucas Moyet, Daniel Salvi, Norbert Rolland, and Daphné Seigneurin-Berny Part IV: Recombinant Protein Production in Yeast 9. High Cell-Density Expression System: Yeast Cells in a Phalanx Efficiently Produce a Certain Range of "Difficult-to-Express" Secretory Recombinant Proteins Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito Part V: Recombinant Protein Production in Insect Cells-Baculovirus 10. Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins Judit Osz-Papai, Laura Radu, Wassim Abdulrahman, Isabelle Kolb-Cheynel, Nathalie Troffer-Charlier, Catherine Birck, and Arnaud Poterszman Part VI: Recombinant Protein Production in Mammalian Cells 11. Transient Expression in HEK 293 Cells: An Alternative to E. coli for the Production of Secreted and Intracellular Mammalian Proteins Joanne E. Nettleship, Peter J. Watson, Nahid Rahman-Huq, Louise Fairall, Mareike G. Posner, Abhishek Upadhyay, Yamini Reddivari, Jonathan M.G. Chamberlain, Simon E. Kolstoe, Stefan Bagby, John W.R. Schwabe, and Raymond J. Owens 12. Recombinant Glycoprotein Production in Human Cell Lines Kamilla Swiech, Marcela Cristina Corrêa de Freitas, Dimas Tadeu Covas, and Virgínia Picanço-Castro Part VII: Recombinant Protein Production in Other Systems 13. Soluble Recombinant Protein Production in Pseudoaltermonas haloplanktis TAC125 Maria Giuliani, Ermenegilda Parrilli, Filomena Sannino, Gennaro Apuzzo, Gennaro Marino, and Maria Luisa Tutino Part VIII: Insoluble Protein Purification 14. A Screening Methodology for Purifying Proteins with Aggregation Problems Mario Lebendiker, Michal Maes, and Assaf Friedler 15. Solubilization and Refolding of Inclusion Body Proteins Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda 16. Bacterial Inclusion Body Purification Joaquin Seras-Franzoso, Spela Peternel, Olivia Cano-Garrido, Antonio Villaverde, and Elena García-Fruitós 17. Characterization of Intracellular Aggresomes by Fluorescent Microscopy Lianwu Fu and Elizabeth Sztul Part IX: Insoluble Protein Characterization 18. Dialysis: A Characterization Method of Aggregation Tendency Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez 19. Applications of Mass Spectrometry to the Study of Protein Aggregation Sílvia Bronsoms and Sebastián A. Trejo 20. Insoluble Protein Assemblies Characterized by Fourier Transform Infrared Spectroscopy Antonino Natalello and Silvia M. Doglia 21. Insoluble Protein Characterization by Circular Dichroism (CD) Spectroscopy and Nuclear Magnetic Resonance (NMR) Shaveta Goyal, Haina Qin, Liangzhong Lim, and Jianxing Song 22. Methods for Characterization of Protein Aggregates Witold Tatkiewicz, Elisa Elizondo, Evelyn Moreno, Cesar Díez-Gil, Nora Ventosa, Jaume Veciana, and Imma Ratera 23. Predicting the Solubility of Recombinant Proteins in Escherichia coli Roger G. Harrison and Miguel J. Bagajewicz Part X: Insoluble Protein Applications 24. Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts Eva Hrabárová, Lucia Achbergerová, and Jozef Nahálka... Mehr