Immunological Tolerance: Methods and Protocols Table of Contents Introduction 1. Frontiers of Immunological Tolerance Giorgio Raimondi, Heth Turnquist & Angus Thomson University of Pittsburgh Medical Center, Thomas E Starzl Transplantation Institute, Pittsburgh, USA Cell Types Contributing to Immunological Tolerance 2. Case Study: Balancing Tolerance & Immunity: The Role of Dendritic Cell & T Cell Subsets Elena Chklovskaia & Barbara Fazekas de St Groth Centenary Institute of Cancer Medicine & Cell Biology, University of Sydney, Sydney, Australia 3. Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow Ludovica Bruno The Institute of Cancer Research, London, UK 4. Genetic Modification of Dendritic Cells through the Directed Differentiation of Embryonic Stem Cells Paul J. Fairchild, Kathleen F. Nolan, &Herman Waldmann Sir William Dunn School of Pathology, University of Oxford, Oxford, UK 5. Generation of Immunocompetent T Cells from Embryonic Stem Cells Renée F. de Pooter & Juan-Carlos Zuñiga-Pflücker Dept of Immunology, University of Toronto, Sunnybrook & Women's Research Institute, Toronto, Canada 6. Isolation, Expansion and Characterization of Human Natural and Adaptive Regulatory T Cells Silvia Gregori, Rosa Bacchetta,Laura Passerini, Megan K. Levings & Maria Grazia Roncarolo San Raffaele Telethon Institute for Gene Therapy, Milan, Italy 7. Derivation, Culture and Characterization of Thymic Epithelial Cell Lines Michiyuki Kasai & Toshiaki Mizuochi Dept of Research on Blood & Biological Product, National Institute of Infectious Diseases, Tokyo, Japan The Study of Immunological Tolerance in Vitro 8. Case Study: Thymus Organogenesis and Development of the Thymic Stroma Craig Scott Nowell, Alison Mary Farley & Catherine Clare Blackburn Institute for Stem Cell Research, University of Edinburgh, Edinburgh, UK 9. Case Study: Generation of a Tissue Engineered Thymic Organoid Fabrizio Vianello & Mark C. Poznansky Infectious Diseases Division & Partners AIDS Research Center, Harvard Medical School, Charlestown, USA 10. Studying T Cell Repertoire Selection Using Fetal Thymus Organ Cultures Philip G. Ashton-Rickardt Department of Pathology & Ben May Institute for Cancer Research, University of Chicago, Chicago, USA 11. Investigating Central Tolerance with Reaggregate Thymus Organ Cultures Graham Anderson & Eric J. Jenkinson MRC Centre for Immune Regulation, Department of Anatomy, University of Birmingham, Birmingham, UK 12. Estimating Thymic Function through Quantification of T Cell Receptor Excision Circles Marie-Lise Dion, Rafick-Pierre Sékaly & Rémi Cheynier Laboratoire d'Immunologie, Centre de Recherches du CHUM, Montréal, Canada 13. Gene Expression Profiling of Dendritic Cells by Microarray Maria Foti, Paola Ricciardi-Castagnoli & Francesca Granucci Dept of Biotechnology and Bioscience, University of Milano-Bicocca, Milan, Italy 14. SAGE Analysis of Cell Types Involved in Tolerance Induction Kathleen F. Nolan, Stephen P. Cobbold & Herman Waldmann Sir William Dunn School of Pathology, University of Oxford, Oxford, UK 15. Analyzing the Physicodynamics of Immune Cells in a 3-D Collagen Matrix Peter Reichardt, Frank Gunzer & Matthias Gunzer German Research Centre for Biotechnology, Braunschweig, Germany The Study of Immunological Tolerance in Vivo 16. Case Study: Aetiology of Autoimmune Disease: How T Cells Escape Self-Tolerance Eli Sercarz & Claudia Raja-Gabaglia Torey Pines Institute for Molecular Studies, San Diego, USA 17. Case Study: Animal Models of Spontaneous Autoimmune Disease: Type 1 Diabetes... Mehr
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