Part I HIV-1 Replication - Early Events1. Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile SystemNicholas E. Webb and Benhur Lee2. Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow CytometryNatasha D. Durham and Benjamin K. Chen3. HIV-1 Capsid Stabilization AssayThomas Fricke and Felipe Diaz-Griffero4. Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core IntegrityJoao I. Mamede and Thomas J. Hope 5. HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP ConcentrationsJoseph A. Hollenbaugh and Baek Kim6. Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral VectorJonathan M.O. Rawson, Christine L. Clouser, and Louis M. ManskyPart II HIV-1 RNA Structure and RNA-Protein Interactions7. Novel Biochemical Tools for Probing HIV RNA StructureJason W. Rausch, Joanna Sztuba-Solinska, Sabrina Lusvarghi, and Stuart F. J. Le Grice 8. Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seqSebla B. Kutluay and Paul D. Bieniasz9. Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular InvestigationsDeepali Singh, Ioana Boeras, Gatikrushna Singh, an d Kathleen Boris-LawriePart III HIV-1 Replication - Post-Integrational Events10. Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 IntegraseJacques J. Kessl, Amit Sharma, and Mamuka Kvaratskhelia 11. Quantification of HIV-1 Gag Localization Within Virus Producer CellsAnnalena La Porte and Ganjam V. Kalpana12. Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro Gabrielle C. Todd and Akira OnoPart IV Studying HIV-1 Replication and Pathogenesis in Animal Models13. Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital MicroscopyRadwa Sharaf, Thorsten R. Mempel, and Thomas T. Murooka14. Modeling HIV-1 Mucosal Transmission and Prevention in Humanized MiceMilena Veselinovic, Paige Charlins, and Ramesh Akkina15. High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and PathogenesisTynisha Thomas, Kieran Seay, Jian Hua Zheng, Cong Zhang, Christina Ochsenbauer, John C. Kappes , and Harris GoldsteinPart V Tools to Study HIV-1 Latency and Pathogenesis16. Measuring the Frequency of Latent HIV-1 in Resting CD4 + T Cells Using a Limiting Dilution Co-Culture AssayGregory M. Laird, Daniel I.S. Rosenbloom, Jun Lai, Robert F. Siliciano, and Janet D. Siliciano17. LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation CompoundsUlrike Jung, Mayumi Takahashi, John J. Rossi, and John C. Burnett18. Improved Methods to Detect Low Levels of HIV Using Antibody-Based TechnologiesEliseo A. Eugenin and Joan W. Berman 19. Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected CellsNigora Mukhamedova, Beda Brichacek, Christina Darwish, Anastas Popratiloff, Dmitri Sviridov, and Michael Bukrinsky 20. The Proteomic Characterization of Plasma or Serum from HIV-Infected PatientsNicole A. Haverland, Lance M. Villeneuve, Pawel Ciborowski, and Howard S. Fox21. Proteomic Characterization of Exosomes from HIV-1-Infected CellsMing Li and Bharat RamratnamPart VI NeuroAIDS22. Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western BlotFabienne Rayne, Solène Debaisieux, Annie Tu, Christophe Chopard, Petra Tryoen, and Bruno Beaumelle 23. Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent AssayTory P. Johnson and Avindra Nath24. Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set UpArthur P. Ruiz and Vinayaka R. Prasad 25. Evaluating the Role of Viral Proteins in... Mehr
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