Part I: Glyco-Engineering of Therapeutic Proteins 1. Engineering of Therapeutic and Diagnostic O-Glycans on Recombinant Mucin-Type Immunoglobulin Fusion Proteins Expressed in CHO Cells Linda Lindberg, Jining Liu, and Jan Holgersson 2. Engineering a Human-Like Glycosylation to Produce Therapeutic Glycoproteins Based on 6-Linked Sialylation in CHO Cells Nassimal El Maï, Sandrine Donadio-Andréi, Chloé Iss, Valérie Calabro, and Catherine Ronin 3. Glycoengineered Pichia-Based Expression of Monoclonal Antibodies Dongxing Zha 4. N-Glycosylation Humanization for Production of Therapeutic Recombinant Glycoproteins in Saccharomyces cerevisiae Christelle Arico, Christine Bonnet, and Christophe Javaud 5. Engineering the Baculovirus Genome to Produce Galactosylated Antibodies in Lepidopteran Cells Sylvie Juliant, Marylêne Lévêque, Pierre Cérutti, Annick Ozil, Sylvie Choblet, Marie-Luce Violet, Marie-Christine Slomianny, Anne Harduin-Lepers, and Martine Cérutti Part II: Glycoanalytics 6. Nano LC Chips MS/MS for the Characterization of N-Glycopeptides Generated from Trypsin Digestion of a Monoclonal Antibody Elsa Wagner-Rousset, Christine Schaeffer-Reiss, Audrey Bednarczyk, Nathalie Corvaïa, Alain Van Dorsselaer, and Alain Beck 7. Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry Marie-Claire Janin-Bussat, Laure Tonini, Céline Huillet, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaïa, and Alain Beck 8. Therapeutic Antibody Glycosylation Analysis: A Contract Research Organization Perspective in the Frame of Batch Release or Comparability Support Arnaud Delobel, Fabrice Cantais, Anicet Catrain, Erell Dereux, and Géry Van Vyncht 9. Mass Spectrometric Analysis of O-Linked Oligosaccharides from Various Recombinant Expression Systems Diarmuid T. Kenny, Stefan Gaunit, Catherine A. Hayes, Anki Gustafsson, Magnus Sjöblom, Jan Holgersson, and Niclas G. Karlsson 10. Assessing Fc Glycan Heterogeneity of Therapeutic Recombinant Monoclonal Antibodies Using NP-HPLC T. Shantha Raju 11. Application of Capillary Electrophoresis in Glycoprotein Analysis Richard R. Rustandi, Carrie Anderson, and Melissa Hamm 12. Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology Grace Chen, Sha Ha, and Richard R. Rustandi 13. Hydrophobic Interaction Chromatography to Analyze Glycoproteins Richard R. Rustandi 14. Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7 Ludovic Landemarre and Eric Duverger 15. Analysis of Monoclonal Antibodies by Sedimentation Velocity Analytical Ultracentrifugation W. Blaine Stine, Jr. Part III: Glycoprotein Complexes Characterization 16. Noncovalent Mass Spectrometry for the Characterization of Antibody/Antigen Complexes Cédric Atmanene, Elsa Wagner-Rousset, Nathalie Corvaïa, Alain Van Dorsselaer, Alain Beck, and Sarah Sanglier-Cianferani 17. Conformational Analysis of Recombinant Monoclonal Antibodies with Hydrogen/Deuterium Exchange Mass Spectrometry Damian Houde and John R. Engen 18. Epitope Mapping of Antibodies by Mass Spectroscopy: A Case Study Victor H. Obungu, Valentina Gelfanova, and Lihua Huang Part IV: PK/PD Assays for Therapeutic Antibodies 19. Evaluation of Antibody-Dependent Cell Cytotoxicity Using Lactate Deshydrogenase (LDH) Measurement Matthieu Broussas, Lucile Broyer, and Liliane Goetsch 20. Evaluation of Complement Dependent Cytotoxicity Using ATP Measurement and C1q / C4b Binding Lucile Broyer, Liliane Goetsch, and Mattthieu Broussas 21. Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG / FcgR Interaction Using SPR-Based Biosensor Technology Thierry Champion and Alain Beck 22. Mass Spectrometry Protocol for the Absolute Quantification of a Monoclonal Antibody in Serum with Immunopurification François Becher, Mathieu Dubois, François Fenaille, and Eric Ezan... Mehr