1. Analysis of Liver Connexin ExpressionUsing Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction Michaël Maes, Joost Willebrords,Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken 2. DNA Methylation Analysis of HumanTissue-Specific Connexin Genes Xiaoming Liu and Canxia Xu 3. Detection of Connexins in Liver Cells UsingSodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis JoostWillebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, and MathieuVinken 4. Immunohisto- andCytochemistry Analysis of Connexins Bruno Cogliati, Michaël Maes, IsabelVeloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara CrespoYanguas, and Mathieu Vinken 5. Small Interfering RNA-MediatedConnexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells Miranda E. Good, Daniela Begandt,Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson 6. Generation and Use of TrophoblastStem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancyand Labor Mark Kibschull, Stephen J. Lye, andOksana Shynlova 7. Identification of Connexin43Phosphorylation and S-Nitrosylationin Cultured Primary Vascular Cells Alexander W. Lohman, Adam C. Straub,and Scott R. Johnstone 8. Preparation of Gap Junctions inMembrane Microdomains for Immunoprecipitation and Mass Spectrometry InteractomeAnalysis Stephanie Fowler, Mark Akins, andSteffany A.L. Bennett 9. Scrape Loading/Dye Transfer Assay Pavel Babica, Iva Sovadinová, and Brad L. Upham 10. Microinjection Technique for Assessment of GapJunction Function Michael D. Fridman, Jun Liu, Yu Sun, and Robert M.Hamilton 11. Electroporation Loading and DyeTransfer: A Safe and Robust Method to Probe Gap Junctional Coupling Elke Decrock, Marijke De Bock,Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert 12.Using Fluorescence Recovery After Photobleaching to Study Gap JunctionalCommunication In Vitro MariaKuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley 13.Tracking Dynamic Gap Junctional Coupling in Live Cells by LocalPhoto-Activation and Fluorescence Imaging SongYang and Wen-Hong Li 14.A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication ofSecond Messengers in Cultured Bone Cells JosephP. Stains and Roberto Civitelli 15.Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Methodfor Studying Gap Junctions and Hemichannels JeganIyyathurai, Bernard Himpens, Geert Bultynck, and Catheleyne D'hondt 16.Establishment of the Dual Whole Cell Recording Patch Clamp Configuration forthe Measurement of Gap Junction Conductance RichardD. Veenstra... Mehr