FOXO Transcription Factors

1. Introduction to FOXO Biology Wolfgang Link Part I: Biochemical and Molecular Methods2. Characterization of microRNAs Regulating FOXO ExpressionDoug Hanniford and Eva Hernando 3. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter Christopher P. Cardozo 4. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein TurnoverThomas Heimbucher and Coleen T. Murphy 5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their ActivityAlba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco 6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant GenesMaría Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos 7. Characterization of FOXO AcetylationShang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam 8. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase AssaysAntonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida 9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target GenesVanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira 10. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target PromotersNeeraj Kumar and Arnab Mukhopadhyay 11. RNAi Mediated Silencing of FoxO FactorsPriyankar Sanphui, Akash Saha, and Subhas Chandra Biswas Part II: Imaging Approaches12. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXOFrancisco Castillo, Thomas A. Mackenzie,  and Bastien Cautain 13. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXOMarta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos 14. Image-based Identification of Chemical Compounds Capable of   Trapping FOXO in the Cell NucleusSusana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link 15. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato Part III: Omics and Bioinformatic Approaches 16. Genome-wide Analysis for Identifying FOXO Protein Binding SitesDong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang 17. Mathematical Modeling of Nuclear Trafficking of  FOXO Transcription FactorsBradford E. Peercy and Martin F. Schneider 18. Following Transcriptome to Uncover FOXO Biological Functions Raymond Liang, Vijay Menon, and Saghi Ghaffari Part IV: Animal Models19. Hydra as Model to Determine the Role of FoxO in LongevityThomas C. G. Bosch 20. Genetic Ablation of FOXO in Mice to Investigate its Physiological Role Hwanhee Oh and Jihye Paik Part V: Human Gentic and Clinical Studies21. Analysis of FOXO3 Gene Polymorphisms Associated with Human LongevityTimothy A Donlon, Philip MC Davy, and Bradley J Willcox 22. Analysis of FOXO3a Gene Polymorphism Associated with AsthmaTejas Shah and Kalpana Joshi... Mehr