Part I: Review and Overview Chapters1. Flower Development in Arabidopsis - There is More to it than Learning your ABCsNathanael Prunet and Thomas Jack2. Flower Development in the Asterid LineageBarry Causier and Brendan Davies3. Grass Flower DevelopmentHiro-Yuki Hirano, Wakana Tanaka, and Taiyo Toriba4. Flower Diversity and Angiosperm DiversificationPamela S. Soltis and Douglas E. Soltis5. Flower Development - Open Questions and Future DirectionsFrank Wellmer, John L. Bowman, Brendan Davies, Cristina Ferrándiz, Jennifer C. Fletcher, Robert G. Franks, Emmanuelle Graciet, Veronica Gregis, Toshiro Ito, Thomas P. Jack, Yuling Jiao, Martin M. Kater, Hong Ma, Elliot M. Meyerowitz, Nathanael Prunet, José Luis Riechmann PART II: Genetic and Phenotypic Analyses6. Genetic Screens for Floral Mutants in Arabidopsis thaliana : Enhancers and SuppressorsThanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won, and Xuemei Chen 7. Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem DevelopmentMona M. Monfared and Jennifer C. Fletcher8. Genetic and Phenotypic Analyses of Petal Development in ArabidopsisJudit Szécsi, Barbara Wippermann and Mohammed Bendahmane9. Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and RiceFang Chang, Zaibao Zhang, Yue Jin, and Hong Ma 10. Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thalianaYingxiang Wang, Zhihao Cheng, Pingli Lu, Ljudmilla Timofejeva, Hong Ma11. Genetic and Phenotypic Analyses of Carpel Development in ArabidopsisVicente Balanzà, Patricia Ballester, Monica Colombo, Chloé Fourquin, Irene Martínez-Fernández, and Cristina FerrándizPart III: Microscopy and Histology12. Microscopic Analysis of Arabidopsis OvulesBalaji Enugutti and Kay Schneitz13. Scanning Electron Microscopy Analysis of Floral DevelopmentRobert G. Franks14. Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral TissueAnna Medzihradszky, Kay Schneitz, and Jan U. Lohmann15. The GUS Reporter System in Flower Development StudiesJanaki S. Mudunkothge and Beth A. KrizekPart IV: Experimental Systems16. A Floral Induction System for the Study of Early Arabidopsis Flower DevelopmentDiarmuid Ó'Maoiléidigh and Frank Wellmer17. Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell TypesG. Venugopala Reddy18. Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing FlowersYing Wang and Yuling Jiao19. Laser-assisted Microdissection Applied to Floral TissuesSamuel E. Wuest and Ueli GrossniklausPartV: Molecular Biology, Genomics, and Systems Biology20. Identification of Arabidopsis Knock-out Lines for Genes of InterestJosé Tomás Matus, Thilia Ferrier, and José Luis Riechmann21. Gene Expression Analysis by Quantitative Real-time PCR for Floral TissuesMariana Bustamante, Jian Jin, Oriol Casagran, Tania Nolan, and José Luis Riechmann 22. Misexpression Approaches for the Manipulation of Flower DevelopmentYifeng Xu, Eng-Seng Gan, and Toshiro Ito 23. Next Generation Sequencing Applied to Flower Development: RNA-SeqJun He and Yuling Jiao24. Next-Generation Sequencing Applied to Flower Development: ChIP-SeqEmmanuelle Graciet, Diarmuid Seosamh Ó'Maoiléidigh, and Frank Wellmer25. Live-Imaging of the Arabidopsis Inflorescence MeristemMarcus G. Heisler and Carolyn Ohno26. Gene Regulatory Network Models for Floral Organ DeterminationEugenio Azpeitia, José Davila-Velderrain, Carlos Villarreal, and Elena R. Alvarez-Buylla... Mehr