Ethylene Signaling

Preface... Table of Contents... Contributing Authors...Part I Analysis of Ethylene Biosynthesis1. Gas Chromatography-Based Ethylene Measurement of Arabidopsis SeedlingsGyeong Mee Yoon and Yi-Chun Chen2. Plant Ethylene Detection Using Laser Based Photo-Acoustic SpectroscopyBram Van de Poel and Dominique Van Der Straeten3. Treatment of Plants with Gaseous Ethylene and Gaseous Inhibitors of Ethylene ActionMark L. Tucker, Joonyup Kim, and Chi-Kuang Wen4. Analysis of 1-Aminocyclopropane-1-Carboxylic Acid Uptake Using a Protoplast SystemWon-Yong Song, Sumin Lee, and Moon-Soo Soh5. Escherichia coli-Based Expression and In Vitro Activity Assay of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase and ACC OxidaseShigeru Satoh and Yusuke Kosugi6. Assay Methods for ACS Activity and ACS Phosphorylation by MAP Kinases In Vitro and In VivoXiaomin Han, Guojing Li, and Shuqun ZhangPart II Analysis of the Ethylene Signaling Pathway7. Analysis of Ethylene Receptors: Ethylene Binding AssaysBrad M. Binder and G. Eric Schaller8. Analysis of Ethylene Receptors: Assay for Histidine Kinase ActivityG. Eric Schaller and Brad M. Binder9. Analysis of Ethylene Receptor Interactions by Co-Immunoprecipitation AssaysZhiyong Gao and G. Eric Schaller10. Localization of the Ethylene-Receptor Signaling Complex to the Endoplasmic Reticulum: Analysis by Two-Phase Partitioning and Density Gradient CentrifugationG. Eric Schaller11. Kinase Assay for CONSTITUTIVE TRIPLE RESPONSE 1 (CTR1) in Arabidopsis thalianaHan Yong Lee and Gyeong Mee Yoon12. Circular Dichroism and Fluorescence Spectroscopy to Study Protein Structure and Protein-Protein Interactions in Ethylene SignalingMareike Kessenbrock and Georg GrothPart III Analysis of Ethylene Responses13. The Triple Response Assay and Its Use to Characterize Ethylene Mutants in ArabidopsisCatharina Merchante and Anna N. Stepanova14. Time-Lapse Imaging to Examine the Growth Kinetics of Arabidopsis Seedlings in Response to EthyleneBrad M. Binder15. Inhibitors of Ethylene Biosynthesis and SignalingG. Eric Schaller and Brad M. Binder16. Analysis of Growth and Molecular Responses to Ethylene in Etiolated Rice SeedlingsBiao Ma and Jin-Song Zhang17. Love Me Not Meter: A Sensor Device for Detecting Petal Detachment Forces in Arabidopsis thaliana18. Effects of Ethylene on Seed Germination of Halophyte Plants under Salt StressWeiqiang Li and Lam-Son Phan Tran19. Assessing Attraction of Nematodes to Host Roots using Pluronic Gel MediumValerie M. Williamson and Rasa Cepulyt -Rakauskien... Mehr