ERK Signaling

1. How Genetics has Helped Piece Together the MAPK Signaling PathwayDariel Ashton-Beaucage and Marc Therrien2. In Vitro Enzyme Kinetics Analysis of EGFRZhihong Wang and Christine Candelora3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast CellsNobuo Yoshimoto and Shun'ichi Kuroda4. Structural Studies of ERK2 Protein ComplexesJohannes F. Weijman, Stefan J. Riedl, and Peter D. Mace5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/ErkTal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg6. Assaying Activation and Subcellular Localization of ERK in Cells and TissuesCarme Caelles, Carles Bayod, and Melisa Morcillo7. Detection and Functional Analysis of SUMO-Modified MEKYuji Kubota and Mutsuhiro Takekawa8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) TagLiu Yang and Alexey Veraksa9. High-Throughput In Vitro Identification of Direct MAPK/Erk SubstratesRona Grossman and Ze'ev Paroush10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGEHidetaka Kosako and Kou Motani11. Analysis of Ras/ERK Compartmentalization by Subcellular FractionationLorena Agudo-Ibañez, Piero Crespo, and Berta Casar12. Cell-Based Assays to Study ERK Pathway/Caveolin1 InteractionsRaffaele Strippoli, Asier Echarri, Miguel Angel del Pozo13. The Nuclear Translocation of ERKDenise A. Berti and Rony Seger14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)Zijian Tang and Chengkai Dai15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET BiosensorsYannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress FibersHiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe17. Co-Culture Activation of MAP Kinase in Drosophila S2 CellsJosefa Steinhauer18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase SignalingMarc K. Saba-El-Leil, Christophe Frémin, and Sylvain Meloche19. 3D Organotypic Culture Model to Study Components of ERK SignalingAthina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk PathwayCarmen G. Lechuga, Lucía Simón-Carrasco, Harrys K.C. Jacob, and Matthias Drosten21. Genome-Wide Analysis of RAS/ERK Signaling TargetsJoshua P. Plotnik and Peter C. Hollenhorst22. Probing Chromatin Modifications in Response to ERK SignalingOzgur Oksuz and Wee-Wei Tee23. Analyzing pERK Activation during Planarian RegenerationSusanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and Francesc Cebrià24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline DevelopmentJessica Jie Chen and Swathi Arur25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed ImagesBomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman26. DrosophilaMarta Forés, Aikaterini Papagianni, Laura Rodríguez-Muñoz, and Gerardo Jiménez27. Analyzing ERK Signal Dynamics during Zebrafish SomitogenesisTakaaki Matsui and Yasumasa Bessho28. Modeling RASopathies with Genetically Modified Mouse ModelsIsabel Hernández-Porrasand Carmen Guerra29. Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling PathwaySung-Young Shin and Lan K. Nguyen... Mehr