Environmental Responses in Plants

PaPart I Tropisms1.11. Hydrotropism: Analysis of the Root Response to a Moisture Gradient Regina Antoni, Daniela Dietrich, Malcolm J. Bennett, and Pedro L. Rodriguez 2. Assessing Gravitropic Responses in Arabidopsis Richard Barker, Benjamin Cox, Logan Silber, Arah Sangari, Amir Assadi, and Patrick Masson 3. Physiological Analysis of Phototropic Responses in Arabidopsis Mathias Zeidler 4. Automatic Chloroplast Movement Analysis Henrik Johansson and Mathias Zeidler 5. Microscopic and Biochemical Visualization of Auxins in Plant Tissues Joshua J. Blakeslee and Angus S. Murphy 6. Immunolocalization of PIN and ABCB Transporters in PlantsNicola Carraro and Wendy A. Peer Part II Photoperiodism and Circasian Rhythms 7. Analysis of Circadian Leaf Movements Niels A. Müller and José M. Jimémez-Gómez 8. Sample Preparation of Arabidopsis Thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes Fernando Andrés, Stefano Torti, Coral Vincent, and George Coupland 9. A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs are Temporally Restricted by the Circadian Clock Amaury de Montaigu, Markus C. Berns, and Geroge Coupland 10. Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hyrbid Screens Using a Transcription Factor ORFeome Ghislain Breton, Steve A. Kay, and José L. Pruneda-Paz 11. Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes Craig G. Simpson, John Fuller, Cristiane P. G. Calixto, Jim McNicol, Clare Booth, John W. S. Brown, and Dorothee Staiger 12. Assessing the Impact of Photosynthetic Sugars in the Arabidopsis Circadian ClockMichael J. Haydon and Alex A. R. Webb 13. Assessing Protein Stability Under Different Light and Circadian Conditions Takatoshi Kiba and Rossana Henriques Part III Abiotic Stress Responses 14. Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought Diego M. Almeida, M. Cecília Almadanim, Tiago Lourenço, Isabel A. Abreu, Nelson J. M. Saibo, and M. Margarida Oliveira 15. Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis Thaliana Urszula Piskurewicz and Luis Lopez-Molina 16. Assessing Tolerance to Heavy-Metal Stress in Arabidopsis Thaliana Seedings Estelle Remy and Paula Duque 17. Assessing Drought Responses Using Thermals Infrared Imaging Ankush Prashar and Hamlyn G. Jones 18. Generating Targeted Gene Knockout Lines in Physcomotrella Patens to Study Evolution of Stress- Responsive Mechanisms Monika Maronova and Maria Kalyna 19. Screening Stress Tolerance Traits in Arabidopsis Cell Cultures Imma P. Salamó, Bogáta Boros, and László Szabados 20. Using Arabidopsis Protoplasts to Study Cellular responses to Environmental Stress Ana Confraria and Elena Baena-González 21. Construction of Artificial miRNAs to Prevent Drought Stress in Solanum Tuberosum Anna Wyrzykowska, Marcin Pieczynski, and Zofia Szweykowska-Kulinska Part IV Plant-Pathogen Interactions 22. Virus-Induced Gene Silencing for Gene Function Studies in BarleyMaria Barciszewska-Pacak, Artur Jarmolowski, and Andrzej Pacak 23. Methods for Long-Term Stable Storage of Colletotrichum SpeciesKei Hiruma and Yusuke Saijo 24. Plant Inoculation with the Fungal Leaf Pathogen Colletotrichum HigginsianumKei Hiruma and Yusuke Saijo 25. Tracing Plant Defense Responses in Roots Upon MAMP/DAMP Treatment Kei Hiruma and Yusuke Saijo 26. Analysis of the Immunity=Related Oxidative Bursts by a Luminol-Based Assay Marco Trujillo 27. Quantitative Analysis of Microbe-Associated-Molecular-Pattern (MAMP)-Induced Ca2+ Transients in Plants Fabian Trempel, Stefanie Ranf, Dierk Scheel, and Justin Lee 28. Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR Stephanie Rausch and Sascha Laubinger 29. Determining Nucleosome Position at Individual Loci after Biotic Stress Using MNase-qPCR Margaux Kaster and Sascha Laubinger 30. Phosphoprotein Enrichment Combined with Phosphopeptide Enrichment to Identify Putative Phosphoproteins During Defense Response in Arabidopsis Thaliana Ines Lassowskat, Wolfgang Hoehenwarter, Justin Lee, and Dierk Scheel2. 2. 1111.... Mehr