Enhancement of xylitol production in Candida tropicalis

{"ID":5409638,"post_title":"Enhancement of xylitol production in Candida tropicalis","title":"Enhancement of xylitol production in Candida tropicalis","post_content":"In this study, gk gene was disrupted from xylitol dehydrogenase gene (XYL2) knockout C. tropicalis strain BSXDH-3. The resulting strain was incapable to grow on glycerol. The cells growth on glycerol was resumed by co-expression of gcy1, 2 and 3 genes from Scheffersomyces stipitis under the control of C. tropicalis glyceraldehydes-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed \"IA-3\", than in BSXDH-3. The pentose phosphate pathway (PPP) is a major basis of NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes, which respectively encode glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed \"PP\", than in BSXDH-3. This is the first report of increased metabolic flux toward glycerol metabolic and PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production.","image":"https:\/\/c5-static.dodax.com\/de-ch\/dp\/OMBGKA85DD5-cc42c03d8d-175-175-7277733707319484131","url":"https:\/\/www.vergleiche.ch\/unterhaltung\/product\/enhancement-of-xylitol-production-in-candida-tropicalis\/","min_price":0,"low_price_merchant_id":0,"deeplink":"","ean":9783847335016,"brand":"undefined"}