Characterization of Nanoparticles Intended for Drug Delivery

Preface Table of Contents... Contributing Authors... Part I Introduction 1. Evaluating Nanomedicines: Obstacles and Advancements Magdalena Swierczewska, Rachael M. Crist, and Scott E. McNeil Part II Sterility and Endotoxin Testing 2. Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test Timothy M. Potter, Barry W. Neun, Anna N. Ilinskaya, and Marina A. Dobrovolskaia 3. Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations Barry W. Neun and Marina A. Dobrovolskaia Part III Physicochemical Characterization 4. Elemental Analysis in Biological Matrices Using ICP-MS Matthew N. Hansen and Jeffrey D. Clogston 5. PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection Mackensie C. Smith and Jeffrey D. Clogston 6. Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis Alpana A. Dongargaonkar and Jeffrey D. Clogston 7. Immunoelectron Microscopy for Visualization of Nanoparticles Sarah R. Anderson, David Parmiter, Ulrich Baxa, and Kunio Nagashima 8. Imaging of Liposomes by Transmission Electron Microscopy Ulrich Baxa Part IV Immunology 9. Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties Barry W. Neun, Anna N. Ilinskaya, and Marina A. Dobrovolskaia 10. In Vitro Assessment of Nanoparticle Effects on Blood Coagulation Timothy M. Potter, Jamie C. Rodriguez, Barry W. Neun, Anna N. Ilinskaya, Edward Cedrone, and Marina A. Dobrovolskaia 11. In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells Timothy M. Potter, Sarah L. Skoczen, Jamie C. Rodriguez, Barry W. Neun, Anna N. Ilinskaya, Edward Cedrone, and Marina A. Dobrovolskaia 12. Assessing NLRP3 Inflammasome Activation by Nanoparticles Bhawna Sharma, Christopher B. McLeland, Timothy M. Potter, Stephan T. Stern, and Pavan P. Adiseshaiah 13. Analysis of Complement Activation by Nanoparticles Barry W. Neun, Anna A. Ilinskaya, and Marina A. Dobrovolskaia 14. Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo Timothy M. Potter, Barry W. Neun, and Marina A. Dobrovolskaia 15. Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles Timothy M. Potter, Barry W. Neun, Jamie C. Rodriguez, Anna N. Ilinskaya, and Marina A. Dobrovolskaia 16. Analysis of Nanoparticle Adjuvant Properties In Vivo Barry W. Neun and Marina A. Dobrovolskaia 17. In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions Timothy M. Potter, Barry W. Neun, and Marina A. Dobrovolskaia 18. Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct Pavan P. Adiseshaiah, Sarah L. Skoczen, Jamie C. Rodriguez, Timothy M. Potter, Krishna Kota, and Stephan T. Stern Part V Drug Release and In Vivo Efficacy 19. Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer Sarah L. Skoczen and Stephan T. Stern 20. Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition Pavan P. Adiseshaiah and Stephan T. Stern... Mehr