1. Common Cell Lines Used to Study Bone Morphogenetic Proteins (BMPs)Jessica Ann Cottrell, Donya Burgess, Sarah Michaels, and Melissa B. Rogers 2. In Vitro and In Vivo Osteogenic Differentiation of Human Adipose-Derived Stromal CellsClement D Marshall, Elizabeth A Brett, Alessandra L Moore, Derrick C Wan, and Michael T Longaker 3. Cell-based Gene Therapy System for Delivering BMPsAustin Dickerson, Eleanor L. Davis, Corinne Sonnet, Alan R. Davis, and Elizabeth A. Olmsted-Davis 4. Generation of Endogenous BMP Transcriptional Reporter Cells through CRISPR/Cas9 Genome Editing Luke D. Hutchinson, Polyxeni Bozatzi, Thomas Macartney, and Gopal P. Sapkota 5. High-Throughput, Biosensor-based Approach to Examine Bone Morphogenetic Protein (BMP) - Receptor Interactions Senem Aykul and Erik Martinez-Hackert 6. Mutagenesis and Imaging studies of BMP Signaling Mechanisms in C. elegans Cathy Savage-Dunn, Ryan J. Gleason, Jun Liu, and Richard W. Padgett 7. Gene Regulation of BMP Ligands in Drosophila Brian G. Stultz and Deborah A. Hursh 8. Using Amphioxus as a Basal Chordate Model to Study BMP Signaling Pathway Luok Wen Yong, Iryna Kozmikova, and Jr-Kai Yu 9. Proteolytic Activation of Bmps: Analysis of Cleavage in Xenopus Oocytes and Embryos Hyung-Seok Kim, Autumn McKnite, and Jan L. Christian 10. Imaging and Quantification of P-Smad1/5 in Zebrafish Blastula and Gastrula Embryos Joseph Zinski, Francesca Tuazon, Yan Huang, Mary Mullins, and David Umulis 11. An Adult Zebrafish Model of Fibrodysplasia Ossificans Progressiva Melissa LaBonty and Pamela C. Yelick 12. Generation and Identification of Genetically Modified Mice for BMP Receptors Jingwen Yang and Yuji Mishina 13. Phenotypic Analyses of Genetically Modified Mice for BMP Receptors Jingwen Yang and Yuji Mishina 14. Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-embedded Tissue Sections Maaike Alkema, Marie-José Goumans, and Boudewijn P.T. Kruithof15. Spatial and Quantitative Detection of BMP Activity in Mouse Embryonic Limb Buds Marcelo Rocha Marques and Jean-Denis Bénazet 16. Pharmacologic Strategies for Assaying BMP Signaling Function Teresa Dinter, Geoffrey A. Bocobo, and Paul B. Yu 17. Bone Morphogenetic Proteins (BMPs) and Bone Regeneration Deboleena Kanjilal and Jessica Ann Cottrell 18. Heterotopic Ossification in Mouse Models of Fibrodysplasia Ossificans Progressiva Salin A. Chakkalakal and Eileen M. Shore 19. Double-Humanized Mouse Model to Study Bone Morphogenetic Protein (BMP) Signaling in Tumor Xenografts Jenna Newman, Rachel NeMoyer, Andrew Zloza, and John Langenfeld... Mehr