1. Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder Sophie S Abby and Eduardo PC Rocha 2. Protein Sorting Prediction Henrik Nielsen 3. Cell FractionationMelissa Petiti, Laetitia Houot, and Denis Duché 4. Defining Lipoprotein Localisation by Fluorescence MicroscopyMaria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée 5. Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive PalmitateNienke Buddelmeijer 6. Defining Membrane Protein Localization by Isopycnic Density GradientsRhys A. Dunstan, Iain D. Hay, and Trevor Lithgow 7. Components Sub-cellular Localisation: Cell Surface ExposureAnna Konovalova 8. Probing Inner Membrane Protein Topology by ProteolysisMaxence S. Vincent and Eric Cascales 9. Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM)Mikhail Bogdanov 10. Defining Membrane Protein Topology using pho-lac Reporter FusionsGouzel Karimova and Daniel Ladant 11. In vivo and in vitro Protein-peptidoglycan InteractionsGang Li and S. Peter Howard 12. Measure of Peptidoglycan Hydrolase ActivityYoann G. Santin and Eric Cascales 13. Protein-protein Interaction: Bacterial Two-Hybrid Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant 14. Protein-protein Interactions: Yeast two-hybridJer-Sheng Linand Erh-Min Lai 15. Protein-protein Interactions: Cytology Two-hybridKrishnamohan Atmakuri 16. Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial MembranesLaureen Logger, Abdelrahim Zoued, and Eric Cascales17. Protein-protein Interactions: Co-immunoprecipitationJer-Sheng Linand Erh-Min Lai 18. Protein-protein Interaction: Tandem Affinity Purification in BacteriaJulie P.M. Viala and Emmanuelle Bouveret 19. Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes Raffaele Ieva 20. Protein-protein Interactions: Pull-down Assays Arthur Louche, Suzana P. Salcedo, and Sarah Bigot 21. Protein-protein interactions: Surface Plasmon ResonanceBadreddine Douzi 22. Assessing Energy-dependent Protein Conformational Changes in the TonB SystemRay A. Larsen 23. Defining Assembly Pathways by Fluorescence MicroscopyAbdelrahim Zouedand Andreas Diepold 24. Large Complexes: Cloning Strategy, Production, and PurificationEric Durand and Roland Lloubes 25. Shearing and Enrichment of Extracellular Type IV PiliAlba Katiria, Gonzalez Rivera, and Katrina T. Forest 26. Blue Native PAGE Analysis of Bacterial Secretion ComplexesSusann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner 27. In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomographyGregor L. Weiss, João M. Medeiros, and Martin Pilhofer 28. Structural Analysis of Protein Complexes by Cryo Electron MicroscopyTiago R. D. Costa, Athanasios Ignatiou, and Elena V. Orlova 29. Bacterial Filamentous Appendages Investigated by Solid-state NMR SpectroscopyBirgit Habenstein and Antoine Loquet 30. Identification of Effectors: Precipitation of Supernatant MaterialNicolas Flaugnatti and Laure Journet 31. Energy Requirements for Protein Secretion via the Flagellar type-III Secretion SystemMarc Erhardt 32. Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony BlotBrent S. Weber, Pek Man Ly, and Mario F. Feldman33. Effector Translocation: Cya Reporter AssaySuma Chakravarthy, Bethany Huot, and Brian H. Kvitko 34. Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter SystemJulie Allombert, Anne Vianney, and Xavier Charpentier 35. Effector Translocation Assay: Differential SolubilizationIrina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota 36. Quantitative Determination of Anti-bacterial Activity during Bacterial Co-cultureJuliana Alcoforado Diniz, Birte Hollmann, and Sarah Coulthurst... Mehr
Preis-Alarm
