1. A Bird's Eye View of the Bacterial LandscapeNatividad Ruiz2. Visualizing the Bacterial Cell Surface: An OverviewHarald Engelhardt3. Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus SystemsNadine S. Henderson and David G. Thanassi4. Experimental Manipulation of the Microbial Functional Amyloid Called CurliYizhou Zhou, Daniel R. Smith, David A. Hufnagel, and Matthew R. Chapman5. Visualization of Gram-Positive Bacterial PiliChungyu Chang, I-Hsiu Huang, Antoni P. Hendrickx, and Hung Ton-That6. Single Cell Microfluidic Studies of Bacterial MotilityAdrien Ducret, Olivier Théodoly, and Tâm Mignot7. Chromatographic Analysis of the Escherichia coli Polysialic Acid CapsuleSusan M. Steenbergen and Eric R. Vimr8. Analysis of Exopolysaccharides in Myxococcus xanthus Using Confocal Laser Scanning MicroscopyWei Hu, Renate Lux, and Wenyuan Shi9. Assessment of Multidrug Efflux Assemblies by Surface Plasmon ResonanceElena B. Tikhonova and Helen I. Zgurskaya10. Fluorescence Microscopy and Proteomics to Investigate Subcellular Localization, Assembly, and Function of the Type II Secretion SystemTanya L. Johnson, Aleksandra E. Sikora, Ryszard A. Zielke, and Maria Sandkvist11. Pore Formation by T3SS Translocators: Liposome Leakage AssayEric Faudry, Caroline Perdu, and Ina Attrée12. Isolation of Bacterial Type IV Machine SubassembliesMayukh K. Sarkar, Seyyed I. Husnain, Simon J. Jakubowski, and Peter J. Christie13. Production and Crystallization of Bacterial Type V Secretion ProteinsHye-Jeong Yeo14. Assembly of Bacterial Outer Membrane ProteinsJan Grijpstra, Martine P. Bos, and Jan Tommassen15. The Outer Membrane of Gram-Negative Bacteria: Lipid A Isolation and CharacterizationJessica V. Hankins, James A. Madsen, Brittany D. Needham, Jennifer S. Brodbelt, and M. Stephen Trent16. Quantitative and Qualitative Preparations of Bacterial Outer Membrane VesiclesHalima Chutkan, Ian MacDonald, Andrew Manning, and Meta J. Kuehn17. In Vitro Peptidoglycan Synthesis Assay with Lipid II SubstrateJacob Biboy, Nhat Khai Bui, and Waldemar Vollmer18. Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes Filipe Carvalho, María Graciela Pucciarelli, Francisco García-del Portillo, Didier Cabanes, and Pascale Cossart19. Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis Anna E. Grzegorzewicz and Mary Jackson20. Protein Disulfide Bond Formation in the Periplasm: Determination of the In Vivo Redox State of Cysteine ResiduesKatleen Denoncin, Valerie Nicolaes, Seung-Hyun Cho, Pauline Leverrier, and Jean-Francois Collet21. Using Reporter Genes and the Escherichia coli ASKA Over-Expression Library in Screens for Regulators of the Gram Negative Envelope Stress ResponseJulia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio22. Isolation of Bacteria Envelope ProteinsShu Quan, Annie Hiniker, Jean-Francois Collet, and James C.A. Bardwell23. Patch Clamp Electrophysiology for the Study of Bacterial Ion Channels in Giant Spheroplasts of E. coliBoris Martinac, Paul R. Rohde, Charles G. Cranfield, and Takeshi Nomura24. Electrophysiological Characterization of Bacterial Pore-Forming Proteins in Planar Lipid BilayersOwen S. Mapingire, Beau Wager, and Anne H. Delcour... Mehr