Autophagy

Part I: Autophagry In Vitro1. Recombinant Expression, Purification and Assembly of p62 FilamentsAbul K. Tarafder, Audrey Guesdon, Tanja Kuhm, and Carsten Sachse 2. Structural Studies of Autophagy-related ProteinsMelanie Schwarten, Oliver H. Weiergräber, Dusan Petrovic, Birgit Strodel, and Dieter Willbold 3. Structural Studies of Mammalian Autophagy Lipidation ComplexKazuto Ohashi, Chinatsu Otomo, Zoltan Metlagel, and Takanori Otomo 4. Structural Studies of Selective Autophagy in YeastAkinori Yamasaki, Yasunori Watanabe and Nobuo N. Noda 5. Biophysical Studies of LC3 Family Proteins                                                                Javier H. Hervás, Zuriñe Antón, and Alicia Alonso   6. Reconstituting Autophagy Initiation from Purified Components Peter Mayrhofer and Thomas Wollert 7. Cell-free Reconstitution of Autophagic Membrane FormationMin Zhang and Liang Ge 8. Use of Peptide Arrays for Identification and Characterization of LIR MotifsMads Skytte Rasmussen, Åsa Birna Birgisdottir and Terje Johansen 9. Studying Autophagic Lysosome Reformation in Cells and by an in vitro Reconstitution SystemYang Chen, Qian Peter Su, and Li Yu 10. Formation of Autophagosomes Coincides With Relaxation of Membrane CurvatureJaime Agudo-Canalejo and Roland L. Knorr  11. Studies of Receptor-Atg8 Interactions during Selective Autophagy Christine Abert and Sascha Martens  Part II: Imaging Autophagy in Tissue Culture12. Correlative Light and Electron Miscopy of Autophagosomes Sigurdur Gudmundsson, Jenny Kahlhofer, Nastassia Baylac, Katri Kallio, and Eeva-Liisa Eskelinen 13. Improved Electron Microscopy (EM) Fixation Methods for Tracking Autophagy-associated Membranes in Cultured Mammalian CellsRitsuko Arai and Satoshi Waguri 14. 3-color Simultaneous Live Imaging of Autophagy-related StructuresMaho Hamasaki, Hiroyuki Ueda, Ouin Kunitaki,  and Tamotsu Yoshimori 15. Correlative Live-cell Imaging and Super-resolution Microscopy of Autophagy Eleftherios Karanasios 16. Methods for Imaging Autophagosome Dynamics in Primary NeuronsAudrey Dong, Vineet Vinay Kulkarni, and Sandra Maday 17. Imaging Autophagy in hiPSC-derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease ResearchStathakos, P., Jimenez-Moreno, N., Crompton, L., Nistor, P, Caldwell, M.A., and Lane, J.D 18. Correlative Light Electron Microscopy to analyze LC3 Proteins in Caenorhabditis elegans embryoCéline Largeau and Renaud Legouis 19. Imaging Non-canonical Autophagy and LC3-associated Phagocytosis in Cultured CellsElise Jacquin, Katherine Fletcher, and Oliver Florey Part III: Assays to Measure (and Screen For) Autophagy and Related Processes20. Measurement of Bulk Autophagy by a Cargo Sequestration AssayNikolai Engedal, Morten Luhr, Paula Szalai, and Per O. Seglen 21. Methods to Detect Loss of Lysosomal Membrane Integrity Sonja Aits 22. Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear StressMaria M. Lazari, Idil Orhon, Patrice Codogno, and Nicolas Dupont 23. Identification and Regulation of Multimeric Protein Complexes in Autophagy via SILAC- based Mass Spectrometry ApproachesStéphanie Kaeser-Pebernard, Britta Diedrich, and Jörn Dengjel 24. Identification and Validation of Novel Autophagy Regulators using an Endogenous Readout siGENOME ScreenMaria New, Tim Van Acker, Ming Jiang, Rebecca Saunders, Jaclyn S. Long, Jun-ichi Sakamaki, Kevin M. Ryan, Michael Howell, and Sharon A. Tooze 25. Autophagy Pathway Mapping to Elucidate the Function of Novel Autophagy Regulators Identified by High-throughput ScreeningMartina Wirth1 and Sharon A. Tooze 26. In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian CellsElena Seranova, Carl Ward, Miruna Chipara, Ratul Banerjee, Tatiana R. Rosenstock, and Sovan Sarkar 27. In vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian CellsElena Seranova, Carl Ward, Miruna Chipara, Ratul Banerjee, Tatiana R. Rosenstock, and Sovan Sarkar 28. Methods for the Study of Entotic Cell DeathJens C. Hamann, Sung Eun Kim, and Michael Overholtzer 29. MHC Class I Internalization via Autophagy ProteinsMonica Loi, Laure-Anne Ligeon and Christian Münz Part IV: Measuring and Imaging Autophagy In Vitro30. Analysis of Autophagy for Liver PathogenesisNazmul Huda, Hui Zou, Shengmin Yan, Bilon Khambu, and Xiao-Ming Yin 31. Autophagy in 3D In Vitro and Ex Vivo Cancer ModelsCarlo Follo, Dario Barbone, William G. Richard2, Raphael Bueno, and V. Courtney Broaddus 32. Autophagy in PlateletsMeenakshi Banerjee , Yunjie Huang, Madhu M. Ouseph , Smita Joshi , Irina Pokrovskaya , Brian Storrie , Jinchao Zhang , Sidney W. Whiteheart , and Qing Jun Wang 33. Methods to Image Macroautophagy in the Brain In VivoXigui Chen, Kanoh Kondo, and Hitoshi Okazawa 34. Measuring Nonselective and Selective Autophagy in the LiverTakashi Ueno and Masaaki Komatsu 35. Measuring Autophagy in PancreatitisAlejandro Ropolo, Daniel Grasso, and Maria I. Vaccaro 36. Characterization of the "autophagic flux" in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal MicroscopyKalamida D., Giatromanolaki A., and Koukourakis M.I 37. Methods to Determine the Role of Autophagy Proteins in C. elegans AgeingSivan Henis-Korenblita and Alicia Meléndez  Part V: Mitophagy and Other Selective Autophagy Pathways38. Investigating Non-selective Autophagy in DrosophilaSzabolcs Takáts, Sarolta Tóth, Gyözö Szenci, and Gábor Juhász 39. Imaging the Dynamics of Mitophagy in Live CellsAndrew S. Moore and Erika L.F. Holzbaur 40. Triggering Mitophagy with Photosensitizers Cheng-Wei Hsieh and Wei Yuan Yang 41. Investigating Mitophagy and Mitochondrial Morphology in vivo using mito-QCThomas G. McWilliams and Ian G. Ganley 42. Assays to Monitor Mitophagy in Drosophila Panagiotis Tsapras, Anne-Claire Jacomin, and Ioannis P.  Nezis 43. Mitophagy Dynamics in Caenorhabditis elegansKonstantinos Palikaras, Eirini Lionaki, and Nektarios Tavernarakis 44. Methods for Studying Mitophagy in YeastPanagiota Kolitsida and Hagai Abeliovich 45. Measuring Anti-bacterial AutophagyKeith B Boyle and  Felix Randow 46. Quantitative Phosphoproteomics of Selective Autophagy ReceptorsThomas Juretschke, Petra Beli, and Ivan Dikic 47. Analysis of Chaperone-mediated AutophagyY.R. Juste and A.M. Cuervo 48. Interactive Autophagy: Monitoring a Novel Form of Selective Autophagy by Macroscopic ObservationsJana Petri and Roland L. Knorr... Mehr