Arthritis Research: Methods and Protocols, Volume I Andrew P. Cope, PhD I. Synovial Joint Morphology, Histopathology, and Immunohistochemistry 1. Imaging Inflamed Synovial Joints Ai Lyn Tan, Helen I. Keen, Paul Emery, and Dennis McGonagle 2. Arthroscopy as a Research Tool: A Review Richard J. Reece 3. Immunohitochemistry of the Inflamed Synovium Martina Gogarty and Oliver FitzGerald 4. In Situ Hybridisation of Synovial Tissue Stefan Kuchen, Christian A. See,ayer, Michel Neidhart, Renate E. Gay, and Steffen Gay 5. Subtractive Hybridization Jörg H. W. Distler, Oliver Distler, Michel Neidhart, and Steffen Gay 6. Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synovium Ulf Müller-Ladner, Martin Judex, Elena Neumann and Steffen Gay 7. Preparation of Mononuclear Cells from Synovial Tissue Jonathan T. Beech and Fionula M. Brennan 8. Quantitative Image Analysis of Synovial Tissue Pascal O. van der Hall, Maarten C. Kraan and Paul Peter Tak II. Cartilage Matrix and Bone Biology 9. Cartilage Histomorphometry Ernst B. Hunziker 10. Image Analysis of Aggrecan Degradation in Articular Cartilage with Formalin-Fixed Samples Barbara Osborn, Yun Bai, Anna H.K. Plaas, and John D. Sandy 11. In Situ Detection of Cell Death in Articular Cartilage Samantha N. Redman, Ilyas M. Khan, Simon R. Tew and Charles W. Archer 12. Measurement of Glycosaminoglycan Release from Cartilage Explants John S. Mort and Peter J. Roughley 13. Assessment of Collagenase Activity in Cartilage Tim E.Cawston and Tanya G. Morgan 14. Assessment of Gelatinase Expression and Activity in Articular Cartilage Rosalind M. Hembry, Susan J. Atkinson and Gillian Murphy 15. Analysis of MT1-MMP Activity Richard D. Evans and YoshifumiItoh 16. Analysis of TIMP Expression and Activity Linda Troeberg and Hideaki Nagase 17. Bone Histomorphology in Arthritis Models Georg Schett and Birgit Tuerk 18. Generation of Osteoclasts in vitro and Assay of Osteoclast Activity Naoyuki Takahashi, Nobuyuki Udagawa, Yasuhiro Kobayashi and Tatsuo Suda III. Cell Trafficking, Migration and Invasion 19. Isolation and Analysis of Large and Small Vessel Endothelial Cells Justin C. Mason, Elaine A Lidington and Helen Yarwood 20. Analysis of Flow Based Adhesion in vitro Oliver Florey and Dorian O. Haskard 21. Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy Gabriela Constantin 22. Angiogenesis in Arthritis - Methodological and Analytical Details Ursula Fearon and Douglas J. Veale 23. Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity Zoltan Szekanecz and Alisa Koch 24. Acquisition, Culture and Phenotyping of Synovial Fibroblasts Sanna Rosengren, David L. Boyle and Gary S. Firestein 25. Genotyping of Synovial Fibroblasts: cDNA Array in Combination with RAP-PCR in Arthritis Elena Neumann, Martin Judex, Steffen Gay and Ulf Müller-Ladner 26. Gene Transfer to Synovial Fibroblast - Methods and Evaluation in the SCID Mouse Model Ingmar Meinecke, Edita Rutkauskaite, Antje Cinski, Ulf Müller-Ladner, Steffen Gay and Thomas Pap 27. In vitro Matrigel Fibroblast Invasion Assay Tanja C.A. Tolboom and Tom W.J. Huizinga 28. Culture and Analysis of Circulating Fibrocytes Timothy E. Quan and Richard Bucala... Mehr