Arabidopsis Protocols

Part I. Growing Arabidopsis 1. Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation and Genetic CrossesLuz Rivero, Randy Scholl, Nicholas Holomuzki, Deborah Crist, Erich Grotewold, and Jelena Brkljacic 2. Using Arabidopsis Related Model Species (ARMS): Growth, Genetic Transformation and Comparative GenomicsGiorgia Batelli, Dong-Ha Oh, Matilde Paino D'Urzo, Francesco Orsini, Maheshi Dassanayake, Jian-Kang Zhu, Hans J. Bohnert, Ray A. Bressan and Albino Maggio 3. Growing Arabidopsis in vitro: Cell Suspensions, in vitro Culture, and RegenerationBronwyn J. Barkla, Rosario Vera-Estrella, and Omar Pantoja Part II. Arabidopsis resources 4. Arabidopsis Database and Stock ResourcesDonghui Li, Kate Dreher, Emma Knee, Jelena Brkljacic, Erich Grotewold, Tanya Z. Berardini, Philippe Lamesch, Margarita Garcia-Hernandez, Leonore Reiser and Eva Huala 5. Bioinformatic Tools in Arabidopsis ResearchMiguel de Lucas, Nicholas J. Provart, and Siobhan Brady Part III. Genetic techniques 6. Exploiting Natural Variation in ArabidopsisJohanna A. Molenaar and Joost J. B. Keurentjes 7. Grafting in ArabidopsisKatherine Bainbridge, Tom Bennett, Peter Crisp, Ottoline Leyser, and Colin Turnbull 8. Agrobacterium tumefaciens Mediated Transient Transformation of Arabidopsis thaliana LeavesSilvina Mangano, Cintia Daniela González, and Silvana Petruccelli 9. iTilling: Personalized Mutation ScreenSusan M. Bush and Patrick J. Krysan 10. Tailor Made Mutations in Arabidopsis Using Zinc-Finger NucleasesYiping Qi, Colby G. Starker, Feng Zhang, Nicholas J. Baltes, and Daniel F. Voytas 11. The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis thalianaAndrew L. Eamens, Marcus McHale, and Peter M. Waterhouse 12. Generation and Identification of Arabidopsis EMS MutantsLi-Jia Qu and Gengi Qin 13. Generation and Identification of Arabidopsis T-DNA Insertion MutantsLi-Jia Qu and Gengi Qin 14. Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation SequencingNaoyuki Uchida, Tomoaki Sakamoto, Masao Tasaka, and Tetsuya Kurata 15. Arabidopsis Transformation with Large Bacterial Artificial Chromosomes Jose M. Alonso and Anna N. Stepanova 16. Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP)Mahmoud W. Yaish, Mingsheng Peng, and Steven J. Rothstein Part IV. Molecular biological techniques 17. Next Generation Mapping of Genetic Mutations Using Bulk Population SequencingRyan S. Austin, Steven P. Chatfield, Darrell Desveaux, and David S. Guttman 18. Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches András Gorzsás and Björn Sundberg 19. A Pipeline for 15N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thalianaBenjamin B. Minkoff, Heather L. Burch, and Michael R. Sussman 20. Gene Expression Profiling Using DNA MicroarraysKionoshin Maruyama, Kazuko Yamaguchi-Shinozaki, and Kazuo Shinozaki 21. Forward Chemical Genetic Screening Hyunmo Choi, Jun-Young Kim, Young Tae Chang, and Hong Gil Nam 22. Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thalianaJong-Myong Kim, Taiko Kim To, Maho Tanaka, Takaho A. Endo, Akihiro Matsui, Junko Ishida, Fiona C. Robertson, Tetsuro Toyoda, and Motoaki Seki Part V. Cell biological techniques 23. Fluorescence MicroscopySebastien Peter, Klaus Harter, and Frank Schleifenbaum 24. Immunocytochemical Fluorescent In Situ Visualization of Proteins in Arabidopsis Yohann Boutté and Markus Grebe 25. High Pressure Freezing and Freeze Substitution of Arabidopsis For Electron MicroscopyJotham R. Austin, II. 26. Applications of Fluorescent Marker Proteins in Plant Cell BiologyMichael R. Blatt and Christopher Grefen 27. Flow Cytometry and Sorting in ArabidopsisDavid W. Galbraith 28. Live Imaging of Arabidopsis Development Daniel von Wangenheim, Gabor Daum, Jan U. Lohmann , Ernst K. Stelzer, and Alexis Maizel 29. Arabidopsis Organelle Isolation and CharacterisationNicolas L. Taylor, Elke Ströher, and A. Harvey Millar Part VI. Biochemical and physiological techniques 30. Analysis of Subcellular Metabolite Distributions within Arabidopsis thaliana Leaf Tissue: A Primer for Subcellular MetabolomicsStephan Krueger, Dirk Steinhauser, Jan Lisec, and Patrick Giavalisco 31. Hormone ProfilingGaetan Glauser, Armelle Vallat, and Dirk Balmer 32. Purification of Protein Complexes and Characterization of Protein-Protein InteractionsKirby N. Swatek, Chris B. Lee, and Jay J. Thelen 33. Protein Fragment Bimolecular Fluorescence Complementation Analysis for the In vivo Study of Protein-Protein Interactions and Cellular Protein Complex LocalizationsRainer Waadt, Kathrin Schlükling, Julian I. Schroeder, and Jörg Kudla 34. The Split Ubiquitin System for the Analysis of Three-Component InteractionsChristopher Grefen 35. RNA-Binding Protein Immunoprecipitation from Whole Cell ExtractsTino Köster and Dorothee Staiger 36. High Throughput Analysis of Protein-DNA Binding AffinityJosé M. Franco-Zorrilla and Roberto Solano... Mehr