Antibody Engineering

Part I: In Silico Methods 1. Antibody Design and Humanization via In Silico Modeling            Vinodh B. Kurella and Reddy Gali 2. Antibody Affinity Maturation by Computational Design            Daisuke Kuroda and Kouhei Tsumoto 3. Use of IMGT® Databases and Tools for Antibody Engineering and Humanization            Marie-Paule Lefranc, François Ehrenmann, Sofia Kossida, Véronique Giudicelli, and Patrice Duroux Part II: Generation of Diversity 4. Construction of Human Naïve Antibody Gene Libraries            Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, and Katia Scotlandi 5. Construction of Synthetic Antibody Libraries            Déborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau 6. Construction of Histidine-Enriched Shark IgNAR Variable Domain Antibody Libraries for the Isolation of pH-Sensitive vNAR Fragments            Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka, and Harald Kolmar 7. Display Technologies for Generation of Ig Single Variable Domains            Vladimir Bobkov, Bas van der Woning, and Hans de Haard 8. A Streamlined Approach for the Construction of Large Yeast Surface Display Fab Antibody Libraries            Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir, Lars Toleikis, Harald Kolmar, Stefan Becker, and Stefan Zielonka Part III: Selections of Lead Candidate 9. Phage Display and Selections on Purified Antigens            Magali Colazet and Patrick Chames 10. Selection of Antibodies to Transiently Expressed Membrane Proteins Using Phage Display            Martina L. Jones, Stephen M. Mahler, and Sumukh Kumble 11. Selection of Antibody Fragments against Structured DNA by Phage Display            Mahdi Zeraati, Marcel E. Dinger, and Daniel Christ 12. Selection of Antibody Fragments by Yeast Display            Nathalie Scholler 13. Rapid Selection of High Affinity Antibody scFv Fragments Using Ribosome Display            Birgit Dreier and Andreas Plückthun 14. In Vitro Selection of Single Domain Antibody (VHH) Using cDNA Display            Naoto Nemoto, Shigefumi Kumachi, and Hidenao Arai 15. Sequencing and Affinity Determination of Antigen-Specific B Lymphocytes from Peripheral Blood            Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ, and Joanne H. Reed Part IV: Production of Recombinant Antibodies 16. Expression of IgG Monoclonals with Engineered Immune Effector Functions            Rodrigo Vazquez-Lombardi, Damien Nevoltris, Romain Rouet, and Daniel Christ 17. An IRES-Mediated Tricistronic Vector for Efficient Generation of Stable, High Level Monoclonal Antibody Producing CHO DG44 Cell Lines            Jessna H.M. Yeo, Mariati, and Yuansheng Yang 18. Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins            Martin Siegemund, Nadine Beha, and Dafne Müller Part V: Fc Engineering 19. Chemoenzymatic Defucosylation of Therapeutic Antibodies for Enhanced Effector Functions Using Bacterial -Fucosidases            Chao Li, Tiezheng Li, and Lai-Xi Wang 20. Fc Glyco- and Fc Protein-Engineering: Design of Antibody Variants with Improved ADCC and CDC Activity            Christian Kellner, Stefanie Derer, Katja Klausz, Sophia Rosskopf, Tim Wirt, Thies Rösner, Anna Otte, Elisa Cappuzzello, and Matthias Peipp 21. Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for High Affinity Interaction with FcRn at pH 6.0            Abhishek Saxena, Bingxin Bai, Shin-Chen Hou, Lianlian Jiang, Tianlei Ying, Shane Miersch, Sachdev S. Sidhu, and Donghui Wu Part VI: Characterization, Optimization, and Innovative Formats 22. Measuring Antibody-Antigen Binding Kinetics Using Surface Plasmon Resonance            Stephen Hearty, Paul Leonard, Hui Ma, and Richard O'Kennedy 23. Parallel Evolution of Antibody Affinity and Thermal Stability for Optimal Biotherapeutic Development            Edward Franklin, Orla Cunningham, and Brian Fennell 24. The Use of Somatic Hypermutation for the Affinity Maturation of Therapeutic Antibodies            Peter M. Bowers, William J. Boyle, and Robert Damoiseaux 25. Selection and Use of Intracellular Antibodies            Sandrine Moutel, Clément Nizak, and Franck Perez 26. Site-Specific Radioactive Labeling of Nanobodies            Maxine Crauwels, Sam Massa, Charlotte Martin, Cecilia Betti, Steven Ballet, Nick Devoogdt, Catarina Xavier, and Serge Muyldermans... Mehr