Antibiotics

Part I: Production and Design1. Antibiotics - Precious Goods in Changing TimesPeter Sass2. Mining Bacterial Genomes for Secondary Metabolite Gene ClustersMartina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert3. Production of Antimicrobial Compounds by FermentationHenrik Harms, Gabriele M. König and Till F. Schäberle4. Structure Elucidation of Antibiotics by NMR SpectroscopyGeorgios Daletos, Elena Ancheeva, Raha Orfali, Victor Wray, and Peter Proksch5. Computer-Aided Drug Design MethodsWenbo Yu and Alexander D. MacKerell, Jr.6. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial AgentsAlexander Zippererand Dorothee KretschmerPart II: Mode of Action7. Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard8. Determination of Bacterial Membrane Impairment by Antimicrobial AgentsMiriam Wilmes and Hans-Georg Sahl9. Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of AnalytesSebastian G. Hoß and Gerd Bendas10. Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and MicroscopyKathi Scheinpflug, Oxana Krylova  and Henrik Strahl11. In vitro Assays to Identify Antibiotics Targeting DNA Metabolism12. Fluorescence-based real-time Activity Assays to Identify RNase P inhibitorsYu Chen, Xin Liu, Nancy Wu, and Carol A. Fierke13. Reporter Gene-based Screening for TPP Riboswitch ActivatorsChristina E. Lünse and Günter Mayer14. Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation Federica Briani15. Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor ScreenMike Gajdiss, Michael Türck, and Gabriele BierbaumPart III: Response and Susceptibility 16. Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory EvolutionShingo Suzuki, Takaaki Horinouchi, and Chikara Furusawa 17. Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research Florian Bonn, Sandra Maass, and Dörte Becher18. Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic ResearchSina Schäkermann, Pascal Prochnow, and Julia E. Bandow19. Functional Metagenomics to Study Antibiotic ResistanceManish Boolchandani, Sanket Patel, and Gautam Dantas20. Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing Miguel Paulo Machado, Bruno Ribeiro-Gonçalves, Mickael Silva, Mário Ramirez, João André Carriço... Mehr