Part I. In Vivtro Models and Assays1. Rapid Generation of Dityrosine Cross-linked Abeta Oligomers via Cu-redox Cycling Adam P. Gunn, Blaine R. Roberts and Ashley I Bush2. Applications of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic ProteinsDahabada H.J. Lopes, Sharmisthia Sinha, Clark Rosenweig, Gal Bitan3. Preparation of Stable Amyloid beta-Protein Oligomers of Defined Assembly OrderClark Rosenweig, Kenjiro One, Kazuma Murakami, Devin K. Lowenstein, Gal Bitan, David B. Teplow4. Purifications and Fibrillation of Full-Length Recombinant PrPNatallia Makarava and Ilia V. Baskakov5. Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism SpectroscopiesMiguel Calero and Maria Gasset 6. Quasielestic Light Scattering Study of Amyloid beta-Protein Fibrillogenesis Aleksey Lomakin and David B. Teplow7. Conformations of Microtuble-Associated Protein Tau Mapped by Fluorescence Resonance Energy Transfer (FRET)Sadasivam Jeganathan, Subashchandrabrose Chinnathamb, Eva-Maria Mandelkow, Echard Mandelkow8. Measuring the Kinetics of Amyloid Fibril Elongation using Quartz Crystal Microbalances (QCM)Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland9. X-ray Fibre Diffraction Studies of Amyloid FibrilsKyle L Morris and Louise C Serpell10.Structural Characterization of Pre-fibrillar Intermediates and Amyloid Fibrils by Small-angle X-ray Scattering Annette Eva Langkilde and Bente Vestergaard11.Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates12. Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy Susanne Wegmann, Daniel J. Muller and Eckhard Mandelkow13. Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR Marçal Vilar, Lei Wang and Roland Riek14.Cyclic Amplification of Prion Protein Misfolding Marcelo A Barria, Dennisse Gonzalez-Romero, and Claudio Soto15. Search for Amyloid Binding Proteins by Affinity Chromatography Miguel Calero, Agueda Rostagno, and Jorge GhisoPart II Cell Culture Models and Assays16. Using Biophysical Approaches for Establishing the Links Between Abeta Aggregation and Cytotoxicity in VitroAsad Jan and Hilal A. Lashuel17. Preparation of Cultured Human Vascular CellsIngvar H. Olafsson, Dadi Th. Vilhjalmsson and Finnbogi R. Thormodsson18. Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells from Mouse BrainSebastien A. Gauthier, Susmita Sahoo, Sonia S. Jung, and Efrat Levy19. In Vitro Assays Measuring Protection by Proteins such as Cystatin C of Primary Cortical Neuronal and Smooth Muscle Cells Sebastein A. Gauthier, Belen Tizon, Susmita Sahoo, and Efrat Levy20. Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids Carlos Villalobos, Erica Caballero, Sara Sanz-Blasco and Lucía Núñez21. Bacterial AmyloidsYizhou Zhou, Luz Blanco, Daniel R. Smith, Matthew R. Chapman 22. Study of Amyloids using YeastReed B. Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey and Herman K. Edskes23. Cell-to-cell Transmission of a-synuclein Aggregate Seung-Jae Lee, Paula Desplats , He-Jin Lee, Brian Spencer and Eliezer MasliahPart III In Vivo Models and Assays24. Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies Per Westermark25. Analysis of S100 Oligomers and AmyloidsHugo M. Botelho, Günter Fritz, Cláudio M. Gomes26. S100A8/A9 Amyloidosis in the Ageing Prostate: Relating ex vivo and in vitro Studies Anna L. Gharibyan, Dina Raveh, Ludmilla A. Morozova-Roche27. Isolation of Amyloid by Solubilization in Water Dadi Th. Vilhjalmsson, Indiana E. Ingolfsdottir and Finnbogi R. Thormodsson 28. Histological Staining of Amyloid and Pre-Amyloid Peptides and Proteins in Mouse Tissue Hameetha B. Rajamohamedsait and Einar M. Sigurdsson29. A Pentameric Luminescent Conjugated Oligothiophene for Optical Imaging of in vitro Formed Amyloid Fibrils and Protein Aggregates in Tissue Sections K. Peter R. Nilsson, Mikael Lindgren and Per Hammarström30. In Vivo Magnetic Resonance Imaging of Amyloid-beta Plaques in Mice Youssef Zaim Wadghiri, Dung Minh Hoang,, Thomas Wisniewski and Einar M. Sigurdsson31 The Mouse Model for Scrapie: Inoculation, Clinical Scoring and Histopathological TechniquesMichele A. Di Bari, Romolo Nonno and Umberto Agrimi32. Biochemical Isolation of Insoluble Tau in Transgenic Mouse Models of Tauopathies Carl Julien and Emmanuel Planel33. Tissue Processing Prior to Analysis of Alzheimer's Disease Associated Proteins and Metabolites, Including Ab Stephen D. Schmidt, Ralph A. Nixon , and Paul M. Mathews34. Ab Measurement by Enzyme-linked Immunosorbent Assay (ELISA) Stephen D. Schmidt, Matthew J. Mazzella, Ralph A. Nixon, and Paul M. Mathews35. Cognitive and Sensorimotor Tasks for Assessing Functional Impairments in Mouse Models of Alzheimer's Disease and Related DisordersAllal Boutajangout, Yong Sheng Li, David Quartermain and Einar M Sigurdsson... Mehr
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