Alpha-Synuclein

Preface...Table of Contents...Contributing Authors...1.Assays for Neuronal Defects Caused by Early Formation of -Synuclein Inclusions in Primary Cultured NeuronsLaura A. Volpicelli-Daley2.Immunohistochemical Detection of Alpha-Synuclein in Unfixed Human Brain TissueJohn B. Sanderson3.Kinetic Measurements of Endocytosis and Exocytosis in Cultured NeuronsKarina Vargas4.Detection of Misfolded -Synuclein Aggregates in Cerebrospinal Fluid by the Protein Misfolding Cyclic Amplification PlatformLuis Concha-Marambio, Mohammad Shahnawaz, and Claudio Soto5.Stereotaxic Targeting of Alpha-Synuclein Pathology in Mouse Brain using Pre-Formed FibrilsBin Zhang, Victoria Kehm, Ron Gathagan, Susan N. Leight, John Q. Trojanowski, Virginia M.-Y. Lee, and Kelvin C. Luk6.Quantification of Alpha-Synuclein in Biological Fluids by Electrochemiluminescence-Based DetectionNiels Kruse and Brit Möllenhauer7.Alpha-Synuclein Proximity Ligation Assay (AS-PLA) in Brain Sections to Probe for Alpha-Synuclein OligomersRosalind F. Roberts, Nora Bengoa-Vergniory, and Javier Alegre-Abarrategui8.Studying -Synuclein Conformation by Intact-Cell CrosslinkingThibaut Imberdis, Saranna Fanning, Andrew Newman, Nagendran Ramalingam, and Ulf Dettmer9.C. elegans as a Model for Synucleinopathies and Other Neurodegenerative Diseases: Tools and TechniquesMandy Koopman, Renée I. Seinstra, and Ellen. A. A. Nollen10.Recombinant Expression and Purification of N-Acetylated Alpha-SynucleinTara A. Eastwood and Daniel P. Mulvihill11.Circular Dichroism and Isothermal Titration Calorimetry to Study the Interaction of -Synuclein with MembranesMatteo Rovere12.Yeast-Based Screens to Target Alpha-Synuclein ToxicityInês Caldeira Brás, Blagovesta Popova, Gerhard H. Braus, and Tiago F. Outeiro13.Probing Structural Changes in Alpha Synuclein by Nuclear Magnetic Resonance SpectroscopyTapojyoti Das and David Eliezer14.Measuring Mitochondrial Dysfunction Caused by Soluble -Synuclein OligomersEric S. Luth and Irina G. Stavrovskaya15.Monitoring -Synuclein Proteotoxicity in Drosophila ModelsAron Szabo and George K. Tofaris16.Detection of -Synuclein in Biological Samples using Mass SpectrometryAnn Brinkmalm, Annika Öhrfelt, Payel Bhattacharjee, and Henrik Zetterberg17.Single-Molecule FRET Detection of Early-Stage Conformations in  -Synuclein AggregationMahdi Muhammad Moosa, Josephine C. Ferreon, and Allan Chris M. Ferreon 18.In Situ Peroxidase Labelling and Mass-Spectrometry of Alpha-Synuclein in Rat Cortical NeuronsRicardo Sanz, Patrick Ovando-Roche, Namrata D. Udeshi, Steven A. Carr, Chee Yeun Chung, and Vikram Khurana19.Probing Alpha-Synuclein Conformations by Electron Paramagnetic Resonance (EPR) SpectroscopyJulia Cattani, Theresa Braun, and Malte Drescher20.Expression and Purification of Untagged  -SynucleinAlex E. Powers and Dushyant S. Patel21.Generation and Analysis of Viral Vector Mediated Rodent Models for Parkinson's DiseaseAkua A. Karikari, James B. Koprich, and Chi Wang Ip... Mehr