undefined, A Thermodynamic Approach to PCR Primer Design,
undefined, A Thermodynamic Approach to PCR Primer Design,

The polymerase chain reaction (PCR), a method for making many copies of a specific DNA fragment, is a fundamental tool of molecular biology. The design of PCR primers, the DNA molecules binding the ends of the fragment to be amplified, is a long standing practical problem crucial for the success of many biological investigations. The book describes the application of principled methods and comprehensive thermodynamic data for predicting DNA binding and folding stabilities to PCR primer design, in... Mehr

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