The Nuclear Envelope: Methods and Protocols

Part I Nuclear Envelope IsolationIsolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope ProteomeNadia Korfali, Laurence Florens, and Eric C. Schirmer Exploring the Protein Composition of the Plant Nuclear EnvelopeXiao Zhou, Kentaro Tamura, Katja Graumann, and Iris Meier High Efficiency Isolation of Nuclear Envelope Protein Complexes from TrypanosomesSamson Obado, Mark C. Field, Brian T. Chait, and Michael P. Rout PartII Nuclear Envelope Protein Interactions, Localization, and Dynamics4.    Super Resolution Microscopy of the Nuclear Envelope and AssociatedProteinsWei Xie, Henning F. Horn, and Graham D. WrightAnalyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS)Shimi Takeshi, Chan-Gi Pack, and Robert D. Goldman Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAPKrishna C. Mudumbi and Weidong Yang The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro Richard D. Byrne, Banafshé Larijani, and Dominic L. Poccia Identifying Protein-Protein Associations at the Nuclear Envelope with BioIDDae In Kim, Samuel C. Jensen, and Kyle J. Roux In Situ Detection of Interactions between Nuclear Envelope Proteins and PartnersAlice Barateau and Brigitte Buendia Methods for Single-Cell Pulse-Chase Analysis of Nuclear ComponentsMarek Drozdz and David J. Vaux Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional ComplementationOlga Tapia and Larry Gerace Analysis of Meiotic Telomere Behavior in the MouseJana Link, Ricardo Benavente, and Manfred Alsheimer PartIII Nuclear Envelope Interactions with the CytoskeletonIdentification and Validation of Putative Nesprin VariantsFlavia Autore, Catherine M. Shanahan, and Qiuping Zhang Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western BlottingJames Carthew and Iakowos Karakesisoglou The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope PropertiesRose-Marie Minaisah, Susan Cox, and Derek T. Warren Cell Microharpooning to Study Nucleo-Cytoskeletal CouplingGregory Fedorchak and Jan Lammerding Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and MyoblastsWakam Chang, Susumu Antoku, and Gregg G. Gundersen Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells Meredith H. Wilson, Matthew G. Bray, and Erika L. F. Holzbaur Imaging Approaches to Investigate Myonuclear Positioning in DrosophilaMafalda Azavedo, Victoria K. Schulman, Eric Folker, Mridula Balakrishnana,  and Mary Baylies PartIV Nuclear Envelope-Chromatin Interactions Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear LaminsAnja R. Oldenburg and Philippe Collas Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion Isabelle Dubant-Goulet DamID Analysis of Nuclear Organization in Caenorhabditis elegans Georgina Gómez-Saldivar, Peter Meister, and Peter Askjaer The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary CellsMichael I. Robson and Eric C. Schirmer Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ HybridizationCraig S. Clements, Ural Bikkul, Mai Hassan Ahmed, Helen A. Foster, Lauren S. Godwin, and Joanna M. Bridger Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 SystemTobias Anton, Heinrich Leonhardt, and Yolanda Markaki Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear LaminaSuzanna Gonzalo and Ray Kreienkamp PartV Nucleo-Cytoplasmic Transport High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore ComplexMartin W. Goldberg An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear MembraneRosemarie Ungricht, Sumit Pawar, and Ulrike Kutay Nuclear Protein Transport in Digitonin Permeabilized CellsSteven A. Adam Analysis of CRM1-Dependent Nuclear Export in Permeabilized CellsRalph H. Kehlenbach and Sarah A. Port SPEED Microscopy and Its Application in Nucleocytoplasmic Transport Jiong Ma, Joseph M. Kelich, and Weidong Yang... Mehr