Part I: Protein Production 1. High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis Yuk Kei Wan, Gigi C.G. Choi, and Alan S.L. Wong 2. Rational Design and Construction of Active-Site Labeled Enzymes Man-Wah Tsang, Yun-Chung Leung, and Kwok-Yin Wong 3. Screening and Production of Recombinant Human Proteins: Ligation Independent Cloning Claire Strain-Damerell, Pravin Mahajan, Alejandra Fernandez-Cid, Opher Gileadi, and Nicola Burgess-Brown 4. Screening and Production of Recombinant Human Proteins: Protein Production in E. coli Nicola A. Burgess-Brown, Pravin Mahajan, Claire Strain-Damerell, Alejandra Fernandez-Cid, Opher Gileadi, and Susanne Gräslund 5. Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells Pravin Mahajan, Claire Strain-Damerell, Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Opher Gileadi, and Nicola A. Burgess-Brown 6. Expression Screening of Human Integral Membrane Proteins Using BacMam Pravin Mahajan, Katherine Ellis, Shubhashish Mukhopadhyay, Alejandra Fernandez-Cid, Gamma Chi, Henry Man, Katharina L. Dürr, and Nicola A. Burgess-Brown 7. High-Throughput Expression Screening in Mammalian Suspension Cells Susan D. Chapple and Michael R. Dyson 8. In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies Lionel Imbert, Rachel Lenoir-Capello, Elodie Crublet, Alicia Vallet, Rida Awad, Isabel Ayala, Celine Juillan-Binard, Hubert Mayerhofer, Rime Kerfah, Pierre Gans, Emeric Miclet, and Jerome Boisbouvier 9. Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes Shah Kamranur Rahman Part II: Structural Analyses and Data Management 10. Hydrogen/Deuterium Exchange-Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins Pui-Kin So 11. BeStSel: From Secondary Structure Analysis to Protein Fold Prediction by Circular Dichroism Spectroscopy András Micsonai, Éva Bulyáki, and József Kardos 12. Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb Andrei L. Turinsky, Sam Dupont, Alexander Botzki, Sabry Razick, Brian Turner, Ian M. Donaldson, and Shoshana J. Wodak 13. State-of-the-Art Data Management: Improving the Reproducibility, Consistency, and Traceability of Structural Biology and In Vitro Biochemical Experiments David Cooper, Marek Grabowski, Matthew D. Zimmerman, Przemyslaw J. Porebski, Ivan G. Shabalin, Magdalena Woinska, Marcin J. Domagalski, Heping Zheng, Piotr Sroka, Marcin Cymborowski, Mateusz P. Czub, Ewa Niedzialkowska, Barat S. Venkataramany, Tomasz Osinski, Zbigniew Fratczak, Jacek Bajor, Juliusz Gonera, Elizabeth MacLean, Kamila Wojciechowska, Wojciech Wajerowicz, Maksymilian Chruszcz, and Wladek Minor Part III: Modeling, Simulation, and Visualization 14. Protein Structure Modeling with MODELLER Benjamin Webb and Andrej Sali 15. Parameterization of a Dioxygen Binding Metal Site Using the MCPB.py Program Pengfei Li and Kenneth M. Merz Jr. 16. Parameterization of Large Ligands for Gromacs Molecular Dynamics Simulation with LigParGen Yu Wai Chen, Yong Wang, Yun-Chung Leung, and Kwok-Yin Wong 17. Simulation of Proteins Modified with a Fluorescent Label Zoe Chan and Yun-Chung Leung 18. Protocol for Simulations of PEGylated Proteins with Martini 3 Fabian Grünewald, Peter C. Kroon, Paulo C.T. Souza, and Siewert J. Marrink 19. Molecular Data Visualization on Mobile Devices: A Quick Starter's Guide Chin-Pang Bennu Yiu and Yu Wai Chen 20. Molecular Data Visualization with Augmented Reality (AR) on Mobile Devices Chin-Pang Bennu Yiu and Yu Wai Chen... Mehr
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