Screening and Optimizing Protein Production in E. coli Lorraine Hewitt and James M. McDonnell Isotopic Labeling of Recombinant Proteins From the Methylotrophic Yeast Pichia pastoris Andrew R. Pickford and Joanne M. O'Leary Perdeuteration/Site-Specific Protonation Approaches for High-Molecular-Weight Proteins Stephen Matthews Segmental Isotopic Labeling for Structural Biological Applications of NMR David Cowburn, Alexander Shekhtman, Rong Xu, Jennifer J. Ottesen, and Tom W. Muir TROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large Proteins Guang Zhu, Youlin Xia, Donghai Lin, and Xiaolian Gao Media for Studies of Partially Aligned States Kieran Fleming and Stephen Matthews Residual Dipolar Couplings in Protein Structure Determination Eva de Alba and Nico Tjandra Projection Angle Restraints for Studying Structure and Dynamics of Biomolecules Christian Griesinger, Wolfgang Peti, Jens Meiler, and Raphael Brüschweiler Characterizing Domain Interfaces by NMR Luke M. Rooney, Sachchidanand, and Jörn M. Werner Characterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic Calculations Jennifer Blake Hall, Olivier Walker, and David Fushman TROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen Bonding Guang Zhu, Youlin Xia, Donghai Lin, and Xiaolian Gao Measurement of Intermediate Exchange Phenomena James G. Kempf and J. Patrick Loria NMR Studies of Partially Folded Molten-Globule States Christina Redfield Structure Determination of Protein Complexes by NMR Daniel Nietlispach, Helen R. Mott, Katherine M. Stott, Peter R. Nielsen, Abarna Thiru, and Ernest D. Laue NMR Studies of Protein-Nucleic Acid Interactions Gabriele Varani, Yu Chen, andThomas C. Leeper Using NMRView to Visualize and Analyze the NMR Spectra of Macromolecules Bruce A. Johnson Automated NMR Structure Calculation With CYANA Peter Güntert NOE Assignment With ARIA 2.0: The Nuts and Bolts Michael Habeck, Wolfgang Rieping, Jens P. Linge, and Michael Nilges Membrane Protein Structure Determination Using Solid-State NMR Anthony Watts, Suzana K. Straus, Stephan L. Grage, Miya Kamihira, Yuen Han Lam, and Xin Zhao Index... Mehr