Pollen and Pollen Tube Biology: Methods and Protocols

Preface...Table of Contents...Contributing Authors...1. Pollen Grain Counting Using a Cell CounterHiroyuki Kakui, Misako Yamazaki, Naoto-Benjamin Hamaya, and Kentaro K. Shimizu2. A Toolkit for teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thalianaHyun Kyung Lee, Stuart Macgregor, and Daphne R. Goring3. Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style PenetrationChrystle Weigand and Jeffrey Harper4. Isolation of the Pistil-Stimulated Pollen Tube SecretomeSaid Hafidh and David Honys5. Restricted Pollination for Tracing Individual Pollen Tubes in a PistilTaro Takahashi, Toshiyuki Mori, and Tomoko Igawa6. Semi-In Vivo Assay for Pollen Tube AttractionSheng Zhong, Zhijuan Wang, and Li-Jia Qu7. Isolation and Cloning of Suppressor Mutants with Improved Pollen FertilitySteven Beuder and Cora A. MacAlister8. Workflow to Characterize Mutants with Reproductive DefectsJennifer A. Noble and Ravishankar Palanivelu9. Internally-Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thalianaDevin K. Smith and Ian S. Wallace10. Arabidopsis thaliana Pollen Tube Culture for Multi-Omics StudiesM. Costa, J. Silva, and S. Coimbra11. Large Scale Analysis of Pollen Viability and Oxidative Level using H2DCFDA-Staining Coupled with Flow CytometryNicholas Rutley and Gad Miller12. Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual-StainingSheng Zhong, Zhijuan Wang, and Li-Jia Qu13. Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular DynamicsFiras Bou Daher and Anja Geitmann14. Analyzing Intracellular Gradients in Pollen TubesDaniel S. C. Damineli, Maria Teresa Portes, and José A. Feijó15. Silicone Chambers for Pollen Tube Imaging in Microstructures In Vitro EnvironmentsHana Bertrand-Rakusová, Youssef Chebli, and Anja Geitmann16. Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen TubesChunxia Zhang, Leonie Steinhorst, and Jörg Kudla17. Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In VitroAna R. Sede, Diego L. Wengier, Cecilia Borassi, José M. Estevez, and Jorge P. Muschietti18. Characterization of Growth Behavior and the Resulting Forces Applied by Pollen Tubes in a 3D MatrixRonny Reimann and Delf Kah19. Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium CompositionTheresa Maria Reimann20. Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and MicroroboticsJan Burri, Gautam Munglani, Bradley J. Nelson, Ueli Grossniklaus, and Hannes Vogler21. Measuring Exocytosis Rate in Arabidopsis Pollen Tube Using Corrected Fluorescence Recovery after Photoconversion (cFRAPc) TechniqueJingzhe Guo and Zhenbiao Yang22. Testing Pollen Tube Proteins for In Vivo Binding to Phosphatidic Acid by n0Butanol Treatment and Confocal MicroscopyCarolin Fritz and Benedikt KostSubject Index List...... Mehr