{"ID":19694183,"title":"Phytochromes: Methods and Protocols"}
Preface...Table of Contents...Contributing Authors...1.Investigation of Light-Regulated Protein-Protein Interactions using Yeast Two-Hybrid AssaysDavid J. Sheerin2.Rapid Examination of Phytochrome-PHYTOCHROME INTERACTING FACTOR (PIF) Interaction by In Vitro Co-Immunoprecipitation AssayInyup Paik and Enamul Huq3.Characterization of Light-Regulated Protein-Protein Interactions by In Vivo Co-Immunoprecipitation (Co-IP) Assays from PlantLing Zhu and Enamul Huq4.Detection of Phytochrome Phosphorylation in PlantsEva Klement, Péter Gyula, and András Viczián5.Detection of SUMOylated Phytochromes in PlantsBeatriz Orosa and András Viczián6.Approaches to Identify Protein Ubiquitination Sites in PlantsFeifei Yu and Qi Xie7.Expression, Purification, and Spectral Characterization of PhytochromesYun-Jeong Han, Jae-Yong Cho, and Jeong-Il Kim8.In Vivo SpectroscopyCornelia Klose9.Basic Phytochrome B CalculationsRobert W. Smith and Christian Fleck10.Quantitative Analysis of PhotobodiesChan Yul Yoo, Desiree Williams, and Meng Chen11.Regulation of Alternative Splicing by PhytochromeTomonao Matsushita12.Phytochrome-Regulation of Seed GerminationKijong Song and Giltsu Choi13.Shade Avoidance and Neighbor DetectionIrma Roig-Villanova, Sandi Paulisic, and Jaime F. Martinez-Garcia14.A Simple Method for Quantification of Protochlorophyllide in Etiolated Arabidopsis SeedlingsMatthew J. Terry and Sylwia M. Kacprzak15.Measuring Phytochrome-Dependant Light-Input to the Plant Circadian ClockRachael J. Oakenfull, James Ronald, and Seth J. Davis16.Characterization of Flowering Time MutantsXu Wang, Qing Liu, Wenjin He, Chentao Lin, and Qin Wang17.Rapid Detection of Hormonal Involvement in Light ResponsesFilip Vandenbussche18.Phytochrome and Light Signaling in MarchantiaKeisuke Inoue, Ryuichi Nishihama, and Takayuki Kohchi19.Analysis of Physcomitrella Phytochrome Mutants via Photo- and PolarotropismAnna Lena Ermert, Fabian Stahl, Tanja Gans, and Jon Hughes20.CRISPR/Cas9-Mediated Knockout of Physcomitrella patens PhytochromesAnna Lena Ermert, Fabien Nogué, Fabian Stahl, Tanja Gans, and Jon Hughes21.Phylogenetic Methods to Study Light SignalingFay-Wei Li and Sarah Mathews...Mehr