Microbial Proteomics

Part I: Global Sample Preparation and Protein Enrichment 1. Filter-Aided Sample Preparation for Proteome Analysis            Jacek R. Wisniewski 2. Protein Enrichment from Highly Dilute Samples with StrataClean            Florian Bonn and Andreas Otto Part II: Subcellular Fractionation 3. Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins            Susanne Sievers 4. Enrichment of Cell Surface-Associated Proteins in Gram+ Bacteria by Biotinylation or Trypsin Shaving for Mass Spectrometry Analysis            Florian Bonn, Sandra Maaß, and Jan Maarten van Dijl 5. Preparation of Bacterial Magnetosomes for Proteome Analysis            Oliver Raschdorf, Dirk Schüler, and René Uebe 6. Analysis of Legionella Metabolism by Pathogen Vacuole Proteomics            Christian Manske, Ivo Finsel, Christine Hoffmann, and Hubert Hilbi Part III: Protein Quantification 7. Detection and Identification of Low-Abundant Proteins Using HPE Gels, Fluorescent Stains, and MALDI-ToF-ToF-MS            Martin Moche, Dirk Albrecht, Reiner Westermeier, and Knut Büttner 8. Applications of Difference Gel Electrophoresis (DIGE) on the Study of Microorganisms            Kathleen Trautwein and Ralf Rabus 9. Proteomic Signatures in Staphylococcus aureus            Susanne Engelmann and Stephan Fuchs 10. How to Assess Protein Stability: Half-Life Determination of a Regulatory Protein in Bacillus subtilis            Alexander Reder, Stephan Michalik, and Ulf Gerth 11. Absolute Protein Quantification Using AQUA-Calibrated 2D-PAGE            Sandra Maa?12. Sulfur-34S and 36S Stable Isotope Labeling of Amino Acids for Quantification (SULAQ34/36) of Proteome Analyses            Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, and Frank Schmidt 13. Metabolic Labeling of Microorganisms with Stable Heavy Nitrogen Isotopes (15N)            Andreas Otto 14. Next-Generation Trapping of Protease Substrates by Label-Free Proteomics            Claudia Lindemann, Nikolas Thomanek, Katja Kuhlmann, Helmut E. Meyer, Katrin Marcus, and Franz Narberhaus 15. In Vivo Proteomics Approaches for the Analysis of Bacterial Adaptation Reactions in Host-Pathogen Settings            Henrike Pförtner, Maren Depke, Kristin Surmann, Frank Schmidt, and Uwe Völker Part IV: Analysis of Post Translational Protein Modifications 16. Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography            Boumediene Soufi, Christoph Täumer, Maja Semanjski, and Boris Macek 17. Phosphoproteomics in Microbiology: Protocols for Studying Streptomyces coelicolor Differentiation            Angel Manteca, Beatriz Rioseras, Adelina Rogowska-Wrzesinska, and Ole N. Jensen 18. Thiol-Redox Proteomics to Study Reversible Protein Thiol Oxidations in Bacteria            Martina Rossius, Falko Hochgräfe, and Haike Antelmann Part V: Metaproteomics 19. Sequential Isolation of DNA, RNA, Protein, and Metabolite Fractions from Murine Organs and Intestinal Contents for Integrated Omics of Host-Microbiota Interactions            Pranjul Shah, Emilie E.L. Muller, Laura A. Lebrun, Linda Wampach, and Paul Wilmes 20. Utilization of a Detergent-Based Method for Direct Microbial Cellular Lysis / Proteome Extraction from Soil Samples for Metaproteomics Studies            Karuna Chourey and Robert L. Hettich 21. Sample Preparation for Metaproteome Analyses of Soil and Leaf Litter            Katharina M. Keiblinger and Katharina Riedel 22. Centrifugation-Based Enrichment of Bacterial Cell Populations for Metaproteomic Studies on Bacteria-Invertebrate Symbioses            Tjorven Hinzke, Manuel Kleiner, and Stephanie Markert... Mehr