Part I: Investigation of Membrane Lipids1. Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass SpectrometryCagakan Özbalci, Timo Sachsenheimer, and Britta Brügger2. Thin-Layer Chromatography of PhospholipidsRania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg3. Analysis of Membrane Lipid Biogenesis Pathways Using Yeast GeneticsMartina Gsell and Günther DaumPart II: Analysis of Structure and Topology of Membrane Proteins4. Using 2D Crystals to Analyze the Structure of Membrane ProteinsIan Collinson, Janet Vonck, and Dilem Hizlan5. Crystallization of Membrane ProteinsFlorian G. Müller and C. Roy D. Lancaster6. Molecular Dynamics Simulations of Membrane ProteinsKristyna Pluhackova, Tsjerk Wassenaar, and Rainer A. Böckmann7. Site-Specific Fluorescent Probe Labeling of Mitochondrial Membrane ProteinsChristine T. Schwall and Nathan N. Alder8. Topology Determination of Untagged Membrane ProteinsIris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner9. self - assembling GFP: A Versatile Tool for Plant (Membrane) Protein AnalysesKatharina Wiesemann, Lucia E. Groß, Manuel Sommer, Enrico Schleiff, and Maik S. SommerPart III: Studying Protein-Protein and Protein-Lipids Interactions within Membranes10. The Use of Cardiolipin-Containing Liposomes as a Model System to Study the Interaction Between Proteins and the Inner Mitochondrial MembraneMilit Marom and Abdussalam Azem11. Analysis of the Interaction Between Membrane Proteins and Soluble Binding Partners by Surface Plasmon ResonanceZht Cheng Wu, Jeanine de Keyzer, Ilja Kusters, and Arnold J.M Driessen12. Peptide Interaction with and Insertion into MembranesRon Saar-Dover, Avraham Ashkenazi, and Yechiel Shai13. Scanning Fluorescence Correlation Spectroscopy in Model Membrane SystemsJoseph D. Unsay and Ana J. García-Sáez14. Analyses of Protein-Protein Interactions by In Vivo Photocrosslinking in Budding YeastTakuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo15. Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane ProteinsAline Le Roy, Hugues Nury, Benjamin Wiseman, Jonathan Sarwan, Jean-Michel Jault, and Christine Ebel16. Membrane Partitioning and Translocation Studied by Isothermal Titration CalorimetryCarolyn Vargas, Johannes Klingler, and Sandro KellerPart IV: Membrane Biogenesis and Dynamics17. Analyzing Membrane Dynamics with Live Cell Fluorescence Microscopy with a Focus on Yeast MitochondriaDirk Scholz, Johannes Förtsch, Stefan Böckler, Till Klecker, and Benedikt Westermann18. Analysis of Protein Translocation into the Endoplasmic Reticulum of Human CellsJohanna Dudek, Sven Lang, Stefan Schorr, Johannes Linxweiler, Markus Greiner, and Richard Zimmermann19. An Assay to Monitor the Membrane Integration of Single-Span ProteinsKatrin Krumpe and Doron Rapaport20. Methods to Study the Biogenesis of Membrane Proteins in Yeast MitochondriaDaniel Weckbecker and Johannes M. HerrmannPart V: Functional Analysis of Membrane Proteins21. Reconstitution of Mitochondrial Presequence Translocase into ProteoliposomesMartin van der Laan, Ralf M. Zerbes, and Chris van der Does22. Single Channel Analysis of Membrane Proteins in Artificial Bilayer MembranesPhilipp Bartsch, Anke Harsman, and Richard Wagner23. Quantification of Protein Complexes by Blue Native ElectrophoresisJuliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, and Ilka Wittig24. Optimizing E. coli -Based Membrane Protein Production Using Lemo21(DE3) and GFP-FusionsAnna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew, and Jan-Willem de Gier... Mehr
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