Preface...Table of Contents...Contributing Authors...1. Overview of Immobilized Enzymes Applications in Pharmaceutical, Chemical, and Food IndustryAlessandra Basso and Simona Serban2. Parameters for the Evaluation of Immobilized Enzymes under Process ConditionsAndrés Illanes and Lorena Wilson3. One-Point Covalent Immobilization of Enzymes on Glyoxyl-Agarose with Minimal Physico-Chemical Modification: Immobilized "Native Enzymes"Jose M. Guisan, Fernando Lopez-Gallego, Juan M. Bolivar, Javier Rocha-Martin, and Gloria Fernandez-Lorente4. Multi-Point Covalent Immobilization of Enzymes on Glyoxyl-Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial EnzymesFernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo, and Jose M. Guisan5. Multi-Potent Covalent Immobilization of Enzymes on Supports Activated with Epoxy Groups: Stabilization of Industrial EnzymesCesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente6. Immobilization of Enzymes on Supports Activated with Glutaraldehyde: A Very Simple Immobilization ProtocolFernando López-Gallego, Jose M. Guisán, and Lorena Betancor7. Very Strong but Reversible Immobilization of Enzymes on Supports Coated with Ionic PolymersCesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente8. Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous MediaGloria Fernandez-Lorente, Javier Rocha-Martin, and Jose M. Guisan9. Immobilization of Enzymes on Hetero-Functional Supports: Physical Adsorption Plus Additional Covalent ImmobilizationLara Trobo-Maseda, Alejandro H. Orrego, María Romero-Fernández, Jose M. Guisan, and Javier Rocha-Martin10. Stabilization of Multimetic Enzymes via Immobilization and Further Cross-Linking with Aldehyde-DextranCesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan11. Immobilization of Enzymes on Magnetic Beads through Affinity InteractionsAudrey Sassolas, Akhtar Hayat, and Jean-Louis Marty12. Immobilization of Proteins on Gold SurfacesJosé M. Abad, Marcos Pita, and Víctor M. Fernández13. Immobilization of Enzymes in Protein FilmsDaniel Sánchez-de Alcázar, Mantas Liutkus, and Aitziber L. Cortajarena14. Encapsulation of Enzymes in Porous Capsules via particle TemplatingMikhail V. Zyuzin, Pedro Ramos-Cabrer, and Susana Carregal-Romero15. Enzyme Immobilization in Wall-Coated Flow MicroreactorsDonya Valikhani, Juan M. Bolivar, and Bernd Nidetzky16. In Situ Immobilization of Enzymes in Biomimetic SilicaErienne Jackson, Sonali Correa, and Lorena Betancor17. Environmentally-Friendly Enzyme Immobilization onto MOF MaterialsVictoria Gascón and Manuel Sánchez-Sánchez18. Co-Immobilization and Co-Localization of Multi-Enzymes Systems on Porous MaterialsAlejandro H. Orrego, Fernando López-Gallego, Gloria Fernandez-Lorente, José M. Guisan, and Javier Rocha-Martin19. Single-Particle Kinetics of Immobilized Enzymes by Harnessing the Autofluorescence of Co-Immobilized CofactorsAna I. Benítez-Mateos 20. Intraparticle pH Sensing within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous ParticlesTanja Consolati, Juan M. Bolivar, Zdenek Petrasek, Jose Berenguer, Aurelio Hidalgo, Jose M. Guisán, and Bernd Nidetzky21. Manufacturing of Protein-Based Biomaterials Coupling Cell-Free Protein Synthesis with Protein ImmobilizationFernando López-Gallego and Ana I. Benítez-Mateos22. Immobilization of Enzymes as Cross-Linked Enzyme Aggregates: General Strategy to Obtain Robust BiocatalystsSusana Velasco-Lozano23. NMR OF Immobilized EnzymesLinda Cerofolini, Enrico Ravera, Marco Fragai, and Claudio Luchinat24. Whole Cell Entrapment TechniquesJorge A. Trelles and Cintia W. Rivero25. Immobilization of INS1E Insulin-Producing Cells within Injectable Alginate HydrogelsAlbert Espona-Noguera, Jesús Ciriza, Alberto Cañibano-Hernández, Laura Saenz del Burgo, and Jose Luis Pedraz26. Immobilization of Cells on Polyurethane FoamIgnacio de Ory, Gema Cabrera, Martin Ramirez, and Ana Blandino27. Covalent Immobilization of Microbial Cells on Microchannel SurfacesGorazd Stojkovic and Polona Znidarsic-Plazl28. Immobilization of Cells by Magnetic NanoparticlesAlireza Ebrahiminezhad, Seyedeh-Masoumeh Taghizadeh, Younes Ghasemi, and Aydin Berenjian29. Cultivation of Productive Biofilms in Flow-Reactors and their Characterization by CLSMChristian David, Ingeborg Heuschkel, Katja Bühler, and Rohan Karande30. Immobilization of MicroalgaeNirupama Mallick31. Therapeutic Applications of Encapsulated CellsArgia Acarregui, Gorka Orive, José Luis Pedraz, and Rosa María Hernández... Mehr
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