GTPase Protocols: The Ras Superfamily

Part I. General Protocols The GTPase Cycle: How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases Edward J. Manser Preparation of GTPases for Structural and Biophysical Analysis Susan J. M. Smith and Katrin Rittinger Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes Arndt A. P. Schmitz, Robert Lucito, and Linda Van Aelst Fluorescence Methods in the Study of Small GTP-Binding Proteins Mohammad Reza Ahmadian, Alfred Wittinghofer, and Christian Herrmann Part II. Specific Methods Ras Family GTPases Ras and Rac as Activators of Reactive Oxygen Species (ROS) Herbert Archer and Dafna Bar-Sagi Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs Hiroshi Maruta, Hong He, and Thao Nheu A Ras-Based Module to Generate 32P-Labeled Fusion Proteins for Blot Overlays Zhoushen Zhao and Louis Lim Rho Family GTPases Determination of the Activity of Rho-Like GTPases in Cells Angeliki Malliri, Jean P. ten Klooster, Cristina Olivo, and John G. Collard Introduction of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases Ivan Tan, Louis Lim, and Thomas Leung Effects of Rho Family GTPases on Cell-Cell Adhesion Masaki Fukata, Masato Nakagawa, Shinya Kuroda, and Kozo Kaibuchi Cell Motility and Invasion Assays Jackie Banyard and Marc Symons Rab Family GTPases Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases Hiroyuki Nakanishi, Takuya Sasaki, and Yoshimi Takai Analysis and Preparation of Stable Complexes between Rab GTPases Rab Escort Protein, and Rab Geranylgeranyl Transferase José B. Pereira-Leal, Anita Q. Gomes, and Miguel C. Seabra Arf Family GTPases Preparation ofMyristoylated Arf1 and Arf6 Proteins Paul A. Randazzo and Henry M. Fales ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins Gustavo Pacheco-Rodriguez, Joel Moss, and Martha Vaughan Arf6 and its Role in Cytoskeletal Modulation Julie G. Donaldson ARF GTPase-Activating Protein 1 Irit Huber, Edna Cukierman, Miriam Rotman, and Dan Cassel Ran GTPases The Use of Permeabilized Cell Systems to Study Nuclear Transport Amy M. Brownawell, James M. Holaska, Ian G. Macara, and Bryce M. Paschal Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes Ralph H. Kehlenbach and Larry Gerace Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton Sofia B. Lizarraga, Yixian Zheng, and Andrew R. Wilde Index... Mehr