1. Site-Specific Conjugation of Thiol-Reactive Cytotoxic Agents to Non-Native Cysteines of Engineered Monoclonal Antibodies Stephan Dickgiesser, Roland Kellner, Harald Kolmar, and Nicolas Rasche 2. Application of Next Generation Maleimides (NGMs) to Site-Selective Antibody Conjugation Maurício Morais, Nafsika Forte, Vijay Chudasama, and James R. Baker 3. Lysine Bioconjugation on Native Albumin with a Sulfonyl Acrylate Reagent Maria J. Matos, Gonzalo Jiménez-Osés, and Gonçalo J.L. Bernardes 4. Engineering Dual-Variable-Domains for the Generation of Site-Specific Antibody-Drug Conjugates Alex R. Nanna and Christoph Rader 5. Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutaminase Jared L. Spidel and Earl F. Albone 6. Engineering Antibodies with C-Terminal Sortase-Mediated Modification for Targeted Nanomedicine Rania A. Hashad, Jaclyn L. Lange, Natasha C.W. Tan, Karen Alt, and Christoph E. Hagemeyer 7. CRISPR/Cas9-Mediated Genetic Engineering of Hybridomas for Creation of Antibodies that Allow Site-Specific Conjugation Makan Khoshnejad, Jacob S. Brenner, Hamideh Parhiz, and Vladimir R. Muzykantov 8. Site-Specific Modification of Proteins via Trypsiligase Sandra Liebscher and Frank Bordusa 9. Site-Selective Functionalization of Nanobodies Using Intein-Mediated Protein Ligation for Innovative Bioconjugation Geert-Jan Graulus, Duy Tien Ta, Huong Tran, Rebekka Hansen, Brecht Billen, Erik Royackers, Jean-Paul Noben, Nick Devoogdt, Serge Muyldermans, Wanda Guedens, and Peter Adriaensens 10. Site-Specific Bioconjugation Using SMARTagâ Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody-Drug Conjugates Junjie Liu, Robyn M. Barfield, and David Rabuka 11. Protein Labeling and Bioconjugation Using N-Myristoyltransferase Karin F.K. Ejendal, Julia G. Fraseur, and Tamara L. Kinzer-Ursem 12. One-Step Fluorescent Protein Labeling by Tubulin Tyrosine Ligase Dominik Schumacher, Heinrich Leonhardt, Christian P.R. Hackenberger, and Jonas Helma 13. Enzymatically Catalyzed Radiofluorination of Biomolecules Christopher R. Drake, Michael J. Evans, and Henry F. VanBrocklin 14. Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase Yi Zhang, Shelby Auger, Jonas V. Schaefer, Andreas Plückthun, and Mark D. Distefano 15. Homogeneous Antibody-Drug Conjugates via Glyco-Engineering Feng Tang, Wei Shi, and Wei Huang 16. Labeling Proteins at Site-Specifically Incorporated 5-Hydroxytryptophan Residues Using a Chemoselective Rapid Azo-Coupling Reaction Partha Sarathi Addy, Sarah B. Erickson, James S. Italia, and Abhishek Chatterjee 17. Facile Site-Specific Multi-Conjugation Strategies in Recombinant Proteins Produced in Bacteria Hannes Merten, Jonas V. Schaefer, Fabian Brandl, Uwe Zangemeister-Wittke, and Andreas Plückthun 18. Site-Specific Photocrosslinking to Immunoglobulin G Using Photoreactive Antibody-Binding Domains Fabiana Zappala and Andrew Tsourkas 19. SpyTag-SpyCatcher Chemistry for Protein Bioconjugation In Vitro and Protein Topology Engineering In Vivo Xiao-Wei Wang and Wen-Bin Zhang 20. FN3 Protein Conjugates for Cancer Diagnosis and Imaging Studies Arutselvan Natarajan and Lotfi Abou-Elkacem... Mehr
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